> Detailanzeige

Couttas, Timothy [Verfasser:in]; Boost, Carola [Verfasser:in]; Pahlisch, Franziska [Verfasser:in]; Sykorova, Eliska [Verfasser:in]; Leweke, Judith E. [Verfasser:in]; Köthe, Dagmar [Verfasser:in]; Endepols, Heike [Verfasser:in]; Rohleder, Cathrin [Verfasser:in]; Leweke, F. Markus [Verfasser:in]

Simultaneous assessment of serum levels and pharmacologic effects of cannabinoids on endocannabinoids and N-acylethanolamines by liquid chromatography-tandem mass spectrometry

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Simultaneous assessment of serum levels and pharmacologic effects of cannabinoids on endocannabinoids and N-acylethanolamines by liquid chromatography-tandem mass spectrometry
  • Beteiligte: Couttas, Timothy [Verfasser:in]; Boost, Carola [Verfasser:in]; Pahlisch, Franziska [Verfasser:in]; Sykorova, Eliska [Verfasser:in]; Leweke, Judith E. [Verfasser:in]; Köthe, Dagmar [Verfasser:in]; Endepols, Heike [Verfasser:in]; Rohleder, Cathrin [Verfasser:in]; Leweke, F. Markus [Verfasser:in]
  • Erschienen: 9 August 2023
  • Erschienen in: Cannabis and cannabinoid research ; 8(2023), 4 vom: Aug., Seite 657-669
  • Sprache: Englisch
  • DOI: 10.1089/can.2021.0181
  • Identifikator:
  • Entstehung:
  • Anmerkungen: Im Titel ist der Buchstabe "N" bei "N-acylethanolamines" in kursiver Schrift geschrieben
  • Beschreibung: Introduction: The primary compounds of Cannabis sativa, delta-9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD), inflict a direct influence on the endocannabinoid system-a complex lipid signaling network with a central role in neurotransmission and control of inhibitory and excitatory synapses. These phytocannabinoids often interact with endogenously produced endocannabinoids (eCBs), as well as their structurally related N-acylethanolamines (NAEs), to drive neurobiological, nociceptive, and inflammatory responses. Identifying and quantifying changes in these lipid neuromodulators can be challenging owing to their low abundance in complex matrices. - Materials and Methods: This article describes a robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the extraction and quantification of the eCBs anandamide and 2-arachidonoylglycerol, along with their congener NAEs oleoylethanolamine and palmitoylethanolamine, and phytocannabinoids CBD, Δ9-THC, and 11-Nor-9-carboxy-Δ9-tetrahydrocannabinol, a major metabolite of Δ9-THC. Our method was applied to explore pharmacokinetic and pharmacodynamic effects from intraperitoneal injections of Δ9-THC and CBD on circulating levels of eCBs and NAEs in rodent serum. - Results: Detection limits ranged from low nanomolar to picomolar in concentration for eCBs (0.012-0.24 pmol/mL), NAEs (0.059 pmol/mL), and phytocannabinoids (0.24-0.73 pmol/mL). Our method displayed good linearity for calibration curves of all analytes (R2>0.99) as well as acceptable accuracy and precision, with quality controls not deviating >15% from their nominal value. Our LC-MS/MS method reliably identified changes to these endogenous lipid mediators that followed a causal relationship, which was dependent on both the type of phytocannabinoid administered and its pharmaceutical preparation. - Conclusion: We present a rapid and reliable method for the simultaneous quantification of phytocannabinoids, eCBs, and NAEs in serum using LC-MS/MS. The accuracy and sensitivity of our assay infer it can routinely monitor endogenous levels of these lipid neuromodulators in serum and their response to external stimuli, including cannabimimetic agents.
  • Zugangsstatus: Freier Zugang