Beschreibung:
This report describes the production and certification of IRMM-311, a reference material of genomic DNA (gDNA) of Bacillus licheniformis DSM 5749 in agarose inserts. This CRM (IRMM-311) is intended to be used for the taxonomic identification of authorised probiotic feed additives by Pulsed Field Gel Electrophoresis (PFGE). The homogeneity and stability of the batch was assessed by monitoring the PFGE pattern of a SfiI restriction enzyme digest of gDNA of Bacillus licheniformis DSM 5749 in agarose inserts. The batch was found to be homogeneous and the material is stable at 4 °C. The batch was characterised by five laboratories determining the PFGE pattern of a SfiI restriction enzyme digest of IRMM-311. The pattern should be considered as a whole and restriction fragments in the size range from 50 kb to 90 kb are certified for their fragment length. DNA sequence analysis of the 3' end of the 16S rRNA gene and the entire 16S-23S internal transcribed spacer (ITS) coding region confirmed the identity of the strain as Bacillus licheniformis. Each vial contains one agarose insert containing gDNA of Bacillus licheniformis DSM 5749.