Beschreibung:
Pt. I. Yeast Cells -- Ch. 1. Freight management in the cell / Johannes M. Herrmann, Anne Spang -- Ch. 2. High-throughput protein extraction and immunoblotting analysis in saccharomyces cerevisiae / Todd C. Lorenz, Vikram C. Anand, Gregory S. Payne -- Ch. 3. Genome-wide analysis of membrane transport using yeast knockout arrays / Helen E. Burston, Michael Davey, Elizabeth Conibear -- Ch. 4. A cell-free system for reconstitution of transport between prevacuolar compartments and vacuoles in saccharomyces cerevisiae / Thomas A. Vida -- Ch. 5. In vitro analysis of the mitochondrial preprotein import machinery using recombinant precursor polypeptides / Dorothea Becker, Martin Krayl, Wolfgang Voos -- Ch. 6. In vitro import of proteins into isolated mitochondria / Karl Bihlmaier, Melanie Bien, Johannes M. Herrmann -- Ch. 7. Synthesis and sorting of mitochondrial translation products / Heike Bauerschmitt, Soledad Funes, Johannes M. Herrmann -- Ch. 8. In vivo labeling and analysis of mitochondrial translation products in budding and in fission yeasts / Karine Gouget, Fulvia Verde, Antoni Barrientos --
Ch. 9. Exploring protein-protein interactions involving newly synthesized mitochondrial DNA encoded proteins / Darryl Horn, Flavia Fontanesi, Antoni Barrientos -- Ch. 10. Purification of yeast membranes and organelles by sucrose density gradient centrifugation / Jennifer Chang, Victoria Ruiz, Ales Vancura -- Ch. 11. Microscopic analysis of lipid droplet metabolism and dynamics in yeast / Heimo Wolinski, Sepp D. Kohlwein -- Ch. 12. Use of bimolecular fluorescence complementation (BiFC) in yeast saccharomyces cerevisiae / Kari-Pekka Skarp, ... [et al.] -- Pt. II. Mammalian cells -- Ch. 13. Using quantitative fluorescence microscopy to probe organelle assembly and membrane trafficking / Brian Storrie, Tregei Starr, Kimberly Forsten-Williams -- Ch. 14. Measuring secretory membrane traffic / Vytaute Starkuviene, ... [et al.] -- Ch. 15. A correlative light and electron microscopy method based on laser micropatterning and etching / Julien Colombelli, ... [et al.] -- Ch. 16. Approaches to investigate the role of signaling in ER-to-Golgi transport / Laura J. Sharpe, Ximing Du, Andrew J. Brown -- Ch. 17. Recruitment of coat proteins to peptidoliposomes / Gregor Suri, Martin Spiess, Pascal Crottet --
Ch. 18. SNAREs mediated fusion of liposomes / Jérôme Vicogne, Jeffrey E. Pessin -- Ch. 19. Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway / Ragna Sannerud, ... [et al.] -- Ch. 20. Tracking the transport of E-cadherin to and from the plasma membrane / Matthew P. Wagoner, Kun Ling, Richard A. Anderson -- Ch. 21. Analysis of nucleocytoplasmic shuttling of NFkB proteins in human leukocytes / Chandra C. Ghosh ... [et al.] -- Ch. 22. Imaging pHluorin-based probes at hippocampal synapses / Stephen J. Royle, ... [et al.] -- Ch. 23. Analysis of receptor tyrosine kinase internalization using flow cytometry / Ning Li, Kristen S. Hill, Lisa A. Elferink -- Ch. 24. Measurement of receptor endocytosis and recycling / Jane M. Knisely, Jiyeon Lee, Guojun Bu -- Ch. 25. Styryl dye-based synaptic vesicle recycling assay in cultured cerebellar granule neurons / Victor Anggono, Michael A. Cousin, Phillip J. Robinson -- Ch. 26. Use of quantitative immunofluorescence microscopy to study intracellular trafficking / Vincent Blot, Timothy E. McGraw -- Ch. 27. Dissecting GLUT4 traffic components in L6 myocytes by fluorescence-based, single cell assays / Costin N. Antonescu, Varinder K Randhawa, Amira Klip -- Ch. 28. Functional genetic analysis of the mammalian mitochondrial DNA encoded peptides / Maria Pilar Bayona-Bafaluy, ... [et al.]