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Beschreibung:
In forest ecosystems, fungal communities are highly diverse since several environmental factors influence their richness and structure. Host plant composition is one of the major factors, as the main input of carbohydrates into soil is plant-derived. Ecological research of fungal communities was hindered by the lack of high-throughput diagnostic tools. To ease the large-scale identification of fungi, we have constructed and validated two generations of ribosomal DNA phylochips. The last generation of developed phylochips carried species-specific probes for about 10,000 fungal species spread over the whole fungal kingdom. We applied the developed phylochips to describe the impact of host trees on ectomycorrhizal communities over the time scale of one year. Furthermore, we monitored the diversity of fungal communities under six different host trees by generating over 180,000 sequences using 454 pyrosequencing approach ...