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Medientyp:
Buch
Titel:
DNA repair protocols
Enthält:
DNA repair : front and center and not going away!
/ Vilhelm A. Bohr
Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome
/ Dashnamoorthy Ravi and Alexander James Roy Bishop
Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening
/ Lone Vedel Schøler, Tine Hørning Møller, Steffen Nørgaard, Lotte Vestergaard, and Anders Olesn
Establishment of the DNA repair-defective mutants in DT40 cells
/ Masamichi Ishiai, Emi Uchida, and Minoru Takata
Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair
/ Chun Tsai, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu
Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair
/ Günter Speit and Andreas Rothfuss
Detection of DNA damage by comet fluorescence in situ hybridization
/ Wiebke Schlörmann and Michael Glei
Quantitative DNA damage and repair measurement with the yeast comet assay
/ Rui Oliveira and Björn Johansson
Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR
/ Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, and Bennett Van Houten
In vitro DNA mismatch repair in human cells
/ Liya Gu, Charles M. Ensor, and Guo-Min Li
Eukaryotic DNA mismatch repair in vitro
/ Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang
Immuno-slot blot assay for detection of UVR-mediated DNA damage
/ Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke
Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay
/ Thomas R. Berton and David L. Mitchell
Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR
/ Ahmad Besaratinia and Gerd P. Pfeifer
Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies
/ Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Schärer
Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts
/ Puck Knipscheer, Markus Räschle, Orlando Schärer, and Johannes C. Walter
In vitro base excision repair using mammalian cell extracts
/ Jason L. Parsons and Grigory L. Dianov
Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro
/ Dolores Córdoba-Cañero, Teresa Roldán-Arjona, and Rafael R. Ariza
In vitro monitoring of base excision repair in Saccharomyces cerevisiae
/ Simone Moertl and Fred Ahne
Mitochondrial base excision repair assays
/ Ricardo Gredilla and Tinna Stevnsner
O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks
/ April M. Luke and Jun Nakamura
DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis
/ Helen E. Bryant
Quantitative amplification of single-stranded DNA
/ Eva-Maria Holstein and David Lydall
Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast
/ James M. Dewar and David Lydall
Monitoring DNA recombination initiated by HO endonuclease
/ Neal Sugawara and James E. Haber
Monitoring I-SceI-induced double-strand break repair in DT40 Cells
/ David du Plessis and Julian E. Sale
I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks
/ Amanda Gunn and Jeremy M. Stark
Studying repair of a single protein-bound nick in vivo using the Flp-nick system
/ Ida Nielsen, Anni H. Andersen, and Lotte Bjergbæk
Measurement of diversification in the immunoglobulin light chain gene of DT40 cells
/ Julian E. Sale
Live cell microscopy of DNA damage response in Saccharomyces cerevisiae
/ Sonia Silva, Irene Gallina, Nadine Eckert-Boulet, and Michael Lisby
Use of gene targeting to study recombination in mammalian cell DNA repair mutants
/ Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn
In vitro rejoining of double strand breaks in genomic DNA
/ George Iliakis, Emil Mladenov, and Nge Cheong
An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides
/ Kamal Datta, Shubhadeep Purkayastha, Ronald D. Neumann, and Thomas A. Winters
Assays of bypass replication of genotoxic lesions in cell-free extracts
/ Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone
Quantitative measurement of translesion DNA synthesis in mammalian cells
/ Omer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel, and Zvi Livneh
Detection of PCNA modifications in Saccharomyces cerevisiae
/ Adelina A. Davies and Helle D. Ulrich
In vitro PCNA modification assays
/ Joanne L. Parker and Helle D. Ulrich
Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication
/ George Iliakis, Emil Mladenov, Ya Wang, and Hong Yan Wang
CHK1 kinase activity assay
/ Hong Yan Wang and Ya Wang
Histone H2AX phosphorylation : a marker for DNA damage
/ Arishya Sharma, Kamini Singh, and Alexandru Almasan.