Bouter, Caroline
[Verfasser:in]
;
Hufert, Frank
[Akademische:r Betreuer:in];
Hufert, Frank
[Sonstige Person, Familie und Körperschaft];
Doenecke, Detlef
[Sonstige Person, Familie und Körperschaft]
The role of NS3 helicase domain in Hepatitis C virus particle assembly
Beschreibung:
Hepatitis C virus non-structural protein 3 (NS3) is a multifunctional protein that obtains serine protease, RNA helicase and NTPase activities. The enzyme is essential for viral RNA replication. Its protease domain cleaves the viral polyprotein at multiple sites. Furthermore, NS3 has recently been implicated to play a role in virus particle assembly. But its function in the assembly process is unknown so far. To determine the role of NS3 in HCV particle assembly alanine scanning mutagenesis within the NS3 helicase domain of chimerical genotype 2a full length replicons was performed. 25 muta...