Ludolphs, Michaela
[VerfasserIn]
;
Steinem, Claudia
[AkademischeR BetreuerIn];
Steinem, Claudia
[Sonstige Person, Familie und Körperschaft];
Köster, Sarah
[Sonstige Person, Familie und Körperschaft]
Spezifität der Wechselwirkung von Collybistin 2 mit Phosphatidylinositolphosphaten: Einfluss der verschiedenen Proteindomänen
Beschreibung:
Collybistin, a brain-specific GEF, is required for gephyrin clustering at the postsynaptic membrane. This is important for the correct arrangement of GABAA and glycine receptors. Binding of collybistin to a membrane is initiated by a C-terminal PH-domain. In general, this domain is known to specifically bind to phosphoinositides (PIPs) in the membrane. The aim of this study was to design an in vitro assay that enables detailed studies of binding of collybistin binding to phosphoinositides. With this assay we analyzed the impact of different collybistin 2 domains on binding to a membrane. So...