Analyzing the eukaryotic translation initiation apparatus and new approaches in affinity chromatography

Medientyp: E-Book; Hochschulschrift

Titel: Analyzing the eukaryotic translation initiation apparatus and new approaches in affinity chromatography

Beteiligte: Seefeldt, Jennifer [Verfasser:in]; Görlich, Dirk [Sonstige Person, Familie und Körperschaft]; Görlich, Dirk [Sonstige Person, Familie und Körperschaft]; Rodnina, Marina [Sonstige Person, Familie und Körperschaft]; Lipka, Volker [Sonstige Person, Familie und Körperschaft]

Erschienen: 2014

Umfang: Online-Ressource

Sprache: Englisch

Identifikator:

Schlagwörter: Hochschulschrift

Entstehung:

Hochschulschrift: Göttingen, Univ., Diss., 2014

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Beschreibung: Eukaryotic translation is a multistep process that utilizes 47 individual translation factor subunits. eIF3 is the largest and most complex translation factor, acting during initiation where it promotes assembly of the 43S preinitiation complex and the recruitment of the mRNA to the ribosome. Here we analyzed the native wheat eIF3 complex, showing that this 13 subunit factor forms a stable complex in vivo. We were able to recombinantly express and purify the individual subunits under native conditions. However they failed to spontaneously assemble into the holocomplex, suggesting that the m...

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