Beschreibung:
Proof-of-concept for the generation of a bioengineered heart muscle (BHM) directly from human pluripotent stem cells (hPSCs) as a model to simulate human heart muscle development was introduced recently by our lab. BHMs undergo stage-specific directed differentiation and self-assembly guided by the sequential addition of growth factors and small molecules to support (1) mesoderm induction (3 days), (2) cardiac specification (10 days) and (3) cardiac maturation (up to 50 days studied). By culture day 22, initial findings revealed homogeneously contracting BHMs with robust inotropic responses...