Böker, Kai Oliver
[VerfasserIn]
;
Gruber, Jens
[AkademischeR BetreuerIn];
Gruber, Jens
[Sonstige Person, Familie und Körperschaft];
Jahn, Reinhard
[Sonstige Person, Familie und Körperschaft]
Functional characterization of npcRNAs - Intercellular trafficking of generegulatory components via exosomes
Beschreibung:
Exosomes are small extracellular vesicles (EVs) of endocytic origin and get released by a multitude of cell types. These vesicles have been found in a variety of body fluids and more recently an important role in exchanging genetic information between cells was postulated for exosomes. The aims of this thesis were to investigate the npcRNA content of exosomes and analyse their putative role in cell-cell communication. I established three different exosome isolation techniques yielding EVs in the appropriate size range determined by nanoparticle tracking analysis (NTA) and detected three exo...