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Medientyp:
Buch
Titel:
Bacterial protein secretion systems
:
methods and protocols
Enthält:
Identification of protein secretion systems in bacterial genomes using MacSyFinder
/ Sophie S. Abby and Eduardo P.C. Rocha
Protein sorting prediction
/ Henrik Nielsen
Cell fractionation
/ Melissa Petiti, Laetitia Houot, and Denis Duché
Defining lipoprotein localisation by fluorescence microscopy
/ Maria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, and Ina Attrée
Identification of lipoproteins using globomycin and radioactive palmitate
/ Nienke Buddelmeijer
Defining membrane protein localization by isopycnic density gradients
/ Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow
Cell surface exposure
/ Anna Konovalova
Probing inner membrane protein topology by proteolysis
/ Maxence S. Vincent and Eric Cascales
Mapping of membrane protein topology by substituted cysteine accessibility method (SCAM)
/ Mikhail Bogdanov
Defining membrane protein topology using pho-lac reporter fusions
/ Gouzel Karimova and Daniel Ladant
In vivo and in vitro protein-peptidoglycan interactions
/ Gang Li and S. Peter Howard
Measure of peptidoglycan hydrolase activity
/ Yoann G. Santin and Eric Cascales
Protein-protein interaction : Bacterial Two-Hybrid
/ Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant
Protein-protein interactions : yeast two-hybrid system
/ Jer-Sheng Lin and Erh-Min Lai
Protein-protein interactions : cytology two-hybrid
/ Krishnamohan Atmakuri
Fusion reporter approaches to monitoring transmembrane helix interactions in bacterial membranes
/ Laureen Logger, Abdelrahim Zoued, and Eric Cascales
Protein-protein interactions : co-immunoprecipitation
/ Jer-Sheng Lin and Erh-Min Lai
Protein-protein interaction : tandem affinity purification in bacteria
/ Julie P.M. Viala and Emmanuelle Bouveret
Site-directed and time-resolved photocrosslinking in cells metabolically labeled with radioisotopes
/ Raffaele Ieva
Protein-protein interactions : pull-down assays
/ Arthur Louche, Suzana P. Salcedo, and Sarah Bigot
Protein-protein interactions : surface plasmon resonance
/ Badreddine Douzi
Assessing energy-dependent protein conformational changes in the TonB system
/ Ray A. Larsen
Defining assembly pathways by fluorescence microscopy
/ Abdelrahim Zoued and Andreas Diepold
Large complexes : cloning strategy, production, and purification
/ Eric Durand and Roland Lloubes
Shearing and enrichment of extracellular type IV pili
/ Alba Katiria Gonzalez Rivera and Katrina T. Forest
Blue native PAGE analysis of bacterial secretion complexes
/ Susann Zilkenat, Tobias Dietsche, Julia V. Monjarás Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner
In situ imaging of bacterial secretion systems by electron cryotomography
/ Gregor L. Weiss, João M. Medeiros, and Martin Pilhofer
Structural analysis of protein complexes by cryo electron microscopy
/ Tiago R.D. Costa, Athanasios Ignatiou, and Elena V. Orlova
Bacterial filamentous appendages investigated by solid-state NMR spectroscopy
/ Birgit Habenstein and Antoine Loquet
Energy requirements for protein secretion via the flagellar type III secretion system
/ Marc Erhardt
Identification of effectors : precipitation of supernatant material
/ Nicolas Flaugnatti and Laure Journet
Screening for secretion of the type VI secretion system protein Hcp by enzyme-linked immunosorbent assay and colony blot
/ Brent S. Weber, Pek Man Ly, and Mario F. Feldman
Effector translocation : cya reporter assay
/ Suma Chakravarthy, Bethany Huot, and Brian H. Kvitko
Monitoring effector translocation using the TEM-1 beta-lactamase reporter system
/ Julie Allombert, Anne Vianney, and Xavier Charpentier
Effector translocation assay : differential solubilization
/ Irina S. Franco, Sara V. Pais, Nuno Charro, and Luís Jaime Mota
Quantitative determination of anti-bacterial activity during bacterial co-culture
/ Juliana Alcoforado Diniz, Birte Hollmann, and Sarah J. Coulthurst.