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Medientyp: E-Artikel; Sonstige Veröffentlichung Titel: Single-use membrane adsorbers for endotoxin removal and purification of endogenous polysialic acid from Escherichia coli K1 Beteiligte: de Vries, Ingo [VerfasserIn]; Schreiber, Sarah [VerfasserIn]; Boßmann, Daniel [VerfasserIn]; Hellmann, Zawadi [VerfasserIn]; Kopatz, Jens [VerfasserIn]; Neumann, Harald [VerfasserIn]; Beutel, Sascha [VerfasserIn] Erschienen: Amsterdam : Elsevier B.V., 2018 Erschienen in: Biotechnology Reports 17 (2018) Ausgabe: published Version Sprache: Englisch DOI: https://doi.org/10.15488/3197; https://doi.org/10.1016/j.btre.2018.02.001 ISSN: 2215-017X Schlagwörter: Article ; DNA determination ; inflammation ; mitochondrial DNA depletion ; ultrafiltration ; glucose intake ; bacterial strain ; anion exchange ; temperature ; priority journal ; optical density ; freeze drying ; cell maturation ; pH ; Single-use ; controlled study ; cell disruption ; fast protein liquid chromatography ; hydrolysis ; coculture ; nonhuman ; Membrane adsorber ; degenerative disease ; Escherichia coli ; [...] Entstehung: Anmerkungen: Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen. Beschreibung: Polysialic acid (polySia) is a promising molecule for various medical applications (e.g., treatment of inflammatory neurodegenerative diseases). In this study a complete production process for human-identical α-(2,8)-linked polySia was developed using a disposable bioreactor for cultivation of Escherichia coli K1 and single-use membrane adsorbers for downstream processing (DSP). The cultivation process was optimized to minimize complex media components and a maturation process after cultivation was established. The maturation led to further product release from the cell surface into the supernatant. Afterwards DSP was established using sodium hydroxide treatment combined with anion exchange membrane adsorbers for endotoxin and DNA depletion. After downstream processing the final product had neither detectable protein nor DNA contamination. Endotoxin content was below 3 EU mg−1. Investigation of the maximal chain length showed no effect of the harsh sodium hydroxide treatment during DSP on the stability of the polySia. Maximal chain length was ∼98 degree of polymerization. © 2018 The Authors Zugangsstatus: Freier Zugang Rechte-/Nutzungshinweise: Namensnennung - Nicht-kommerziell - Keine Bearbeitung (CC BY-NC-ND)