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Vanz, Ana Leticia [VerfasserIn]; Lünsdorf, Heinrich [VerfasserIn]; Adnan, Ahmad [VerfasserIn]; Nimtz, Manfred [VerfasserIn]; Gurramkonda, Chandrasekhar [VerfasserIn]; Khanna, Navin [VerfasserIn]; Rinas, Ursula [VerfasserIn]

Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: Catabolic adaptation, stress responses, and autophagic processes - [published Version]

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  • Medientyp: E-Artikel; Sonstige Veröffentlichung
  • Titel: Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface antigen: Catabolic adaptation, stress responses, and autophagic processes
  • Beteiligte: Vanz, Ana Leticia [VerfasserIn]; Lünsdorf, Heinrich [VerfasserIn]; Adnan, Ahmad [VerfasserIn]; Nimtz, Manfred [VerfasserIn]; Gurramkonda, Chandrasekhar [VerfasserIn]; Khanna, Navin [VerfasserIn]; Rinas, Ursula [VerfasserIn]
  • Erschienen: London : BioMed Central Ltd., 2012
  • Erschienen in: Microbial Cell Factories 11 (2012)
  • Ausgabe: published Version
  • Sprache: Englisch
  • DOI: https://doi.org/10.15488/638; https://doi.org/10.1186/1475-2859-11-103
  • ISSN: 1475-2859
  • Schlagwörter: cell vacuole ; Autophagy ; Vacuoles ; Unfolded Protein Response ; reactive oxygen metabolite ; Recombinant Proteins ; cell size ; Fungal Proteins ; Hepatitis B Surface Antigens ; mitochondrion ; Endoplasmic Reticulum-Associated Degradation ; Eukaryota ; protein degradation ; article ; nonhuman ; methanol ; cell structure ; Reactive Oxygen Species ; oxidative stress ; ER stress ; Hepatitis B virus ; Aox1 promoter ; hepatitis B surface antigen ; alcohol oxidase ; [...]
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  • Beschreibung: Background: Pichia pastoris is an established eukaryotic host for the production of recombinant proteins. Most often, protein production is under the control of the strong methanol-inducible aox1 promoter. However, detailed information about the physiological alterations in P. pastoris accompanying the shift from growth on glycerol to methanol-induced protein production under industrial relevant conditions is missing. Here, we provide an analysis of the physiological response of P. pastoris GS115 to methanol-induced high-level production of the Hepatitis B virus surface antigen (HBsAg). High product titers and the retention of the protein in the endoplasmic reticulum (ER) are supposedly of major impact on the host physiology. For a more detailed understanding of the cellular response to methanol-induced HBsAg production, the time-dependent changes in the yeast proteome and ultrastructural cell morphology were analyzed during the production process.Results: The shift from growth on glycerol to growth and HBsAg production on methanol was accompanied by a drastic change in the yeast proteome. In particular, enzymes from the methanol dissimilation pathway started to dominate the proteome while enzymes from the methanol assimilation pathway, e.g. the transketolase DAS1, increased only moderately. The majority of methanol was metabolized via the energy generating dissimilatory pathway leading to a corresponding increase in mitochondrial size and numbers. The methanol-metabolism related generation of reactive oxygen species induced a pronounced oxidative stress response (e.g. strong increase of the peroxiredoxin PMP20). Moreover, the accumulation of HBsAg in the ER resulted in the induction of the unfolded protein response (e.g. strong increase of the ER-resident disulfide isomerase, PDI) and the ER associated degradation (ERAD) pathway (e.g. increase of two cytosolic chaperones and members of the AAA ATPase superfamily) indicating that potential degradation of HBsAg could proceed via the ERAD pathway and through the ...
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