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Medientyp:
E-Artikel
Titel:
Direct electron irradiation of DNA in fully aqueous environment. Damage determination in combination with Monte Carlo simulations
Beteiligte:
Hahn, Marc Benjamin
[VerfasserIn];
Meyer, Susann
[VerfasserIn];
Schröter, Maria-Astrid
[VerfasserIn];
Seitz, H.
[VerfasserIn];
Kunte, Hans-Jörg
[VerfasserIn];
Solomun, Tihomir
[VerfasserIn];
Sturm, Heinz
[VerfasserIn]
Erschienen:
BAM-Publica - Publikationsserver der Bundesanstalt für Materialforschung und -prüfung (BAM), 2017
Sprache:
Englisch
DOI:
https://doi.org/10.1039/C6CP07707B
Entstehung:
Anmerkungen:
Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
Beschreibung:
We report on a study in which plasmid DNA in water was irradiated with 30 keV electrons generated by a scanning electron microscope and passed through a 100 nm thick Si3N4 membrane. The corresponding Monte Carlo simulations suggest that the kinetic energy spectrum of the electrons throughout the water is dominated by low energy electrons (<100 eV). The DNA radiation damage, single-strand breaks (SSB) and double-strand breaks (DSB), was determined by electrophoresis. The median lethal dose of D1/2 = 1.7 ± 0.3 Gy was found to be much smaller compared to partially or fully hydrated DNA irradiated under vacuum conditions. The ratio of DSB to SSB was found to be (1:12) as compared to 1:88) found for hydrated DNA. Our method enables quantitative measurements of radiation damage to biomolecules (DNA, proteins) in solutions under varying conditions (pH, salinity, cosolutes) for an electron energy range which is difficult to probe by standard methods.