Hobmeier, K.
[VerfasserIn];
Cantone, M.
[VerfasserIn];
Nguyen, Q. A.
[VerfasserIn];
Pflüger-Grau, K.
[VerfasserIn];
Kremling, A.
[VerfasserIn];
Kunte, Hans-Jörg
[VerfasserIn];
Pfeiffer, F.
[VerfasserIn];
Marin-Sanguino, A.
[VerfasserIn]
Adaptation to varying salinity in Halomonas elongata: Much more than ectoine accumulation
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Medientyp:
E-Artikel
Titel:
Adaptation to varying salinity in Halomonas elongata: Much more than ectoine accumulation
Beteiligte:
Hobmeier, K.
[VerfasserIn];
Cantone, M.
[VerfasserIn];
Nguyen, Q. A.
[VerfasserIn];
Pflüger-Grau, K.
[VerfasserIn];
Kremling, A.
[VerfasserIn];
Kunte, Hans-Jörg
[VerfasserIn];
Pfeiffer, F.
[VerfasserIn];
Marin-Sanguino, A.
[VerfasserIn]
Erschienen:
BAM-Publica - Publikationsserver der Bundesanstalt für Materialforschung und -prüfung (BAM), 2022
Sprache:
Englisch
DOI:
https://doi.org/10.3389/fmicb.2022.846677
Entstehung:
Anmerkungen:
Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
Beschreibung:
The halophilic γ-proteobacterium Halomonas elongata DSM 2581T thrives at salt concentrations well above 10 % NaCl (1.7 M NaCl). A well-known osmoregulatory mechanism is the accumulation of the compatible solute ectoine within the cell in response to osmotic stress. While ectoine accumulation is central to osmoregulation and promotes resistance to high salinity in halophilic bacteria, ectoine has this effect only to a much lesser extent in non-halophiles. We carried out transcriptome analysis of H. elongata grown on two different carbon sources (acetate or glucose), and low (0.17 M NaCl), medium (1 M), and high salinity (2 M) to identify additional mechanisms for adaptation to high saline environments. To avoid a methodological bias, the transcripts were evaluated by applying two methods, DESeq2 and Transcripts Per Million (TPM). The differentially transcribed genes in response to the available carbon sources and salt stress were then compared to the transcriptome profile of Chromohalobacter salexigens, a closely related moderate halophilic bacterium. Transcriptome profiling supports the notion that glucose is degraded via the cytoplasmic Entner-Doudoroff pathway, whereas the Embden-Meyerhoff-Parnas pathway is employed for gluconeogenesis. The machinery of oxidative phosphorylation in H. elongata and C. salexigens differs greatly from that of non-halophilic organisms, and electron flow can occur from quinone to oxygen along four alternative routes. Two of these pathways via cytochrome bo' and cytochrome bd quinol oxidases seem to be upregulated in salt stressed cells. Among the most highly regulated genes in H. elongata and C. salexigens are those encoding chemotaxis and motility proteins, with genes for chemotaxis and flagellar assembly severely downregulated at low salt concentrations. We also compared transcripts at low and high-salt stress (low growth rate) with transcripts at optimal salt concentration and found that the majority of regulated genes were down-regulated in stressed cells, including many genes ...