QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Medientyp: E-Artikel

Titel: QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Beteiligte: Nelson, G. [VerfasserIn]; Boehm, U. [VerfasserIn]; Bagley, S. [VerfasserIn]; Bajcsy, P. [VerfasserIn]; Bischof, J. [VerfasserIn]; Brown, C. M. [VerfasserIn]; Dauphin, A. [VerfasserIn]; Dobbie, I. M. [VerfasserIn]; Eriksson, J. E. [VerfasserIn]; Faklaris, O. [VerfasserIn]; Fernandez-Rodriguez, J. [VerfasserIn]; Ferrand, A. [VerfasserIn]; Gelman, L, [VerfasserIn]; Gheisari, A. [VerfasserIn]; Hartmann, H. [VerfasserIn]; Kukat, C. [VerfasserIn]; Laude, A. [VerfasserIn]; Mitkovski, M. [VerfasserIn]; Munck, S. [VerfasserIn]; North, A. J. [VerfasserIn]; Rasse, T. [VerfasserIn]; Resch-Genger, Ute [VerfasserIn]; Schuetz, L. C. [VerfasserIn]; Seitz, A. [VerfasserIn]; [...]

Erschienen: BAM-Publica - Publikationsserver der Bundesanstalt für Materialforschung und -prüfung (BAM), 2021

Sprache: Englisch

DOI: https://doi.org/10.1111/jmi.13041

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Beschreibung: A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated, quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing and quantifying scientific phenomena, neither a thorough understanding of the principles underlying quantitative imaging techniques nor appropriate knowledge of how to calibrate, operate and maintain microscopes can be taken for granted. This is clearly demonstrated by the well-documented and widespread difficulties that are routinely encountered in evaluating acquired data and reproducing scientific experiments. Indeed, studies have shown that more than 70% of researchers have tried and failed to repeat another scientist’s experiments, while more than half have even failed to reproduce their own experiments1. One factor behind the reproducibility crisis of experiments published in scientific journals is the frequent underreporting of imaging methods caused by a lack of awareness and/or a lack of knowledge of the applied technique2,3. Whereas quality control procedures for some methods used in biomedical research, such as genomics (e.g., DNA sequencing, RNA-seq) or cytometry, have been introduced (e.g. ENCODE4), this issue has not been tackled for optical microscopy instrumentation and images. Although many calibration standards and protocols have been published, there is a lack of awareness and agreement on common Standards and guidelines for quality assessment and reproducibility5. In April 2020, the QUality Assessment and REProducibility for instruments and images in Light Microscopy (QUAREP-LiMi) initiative6 was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ...

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