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Chen, Xi [Verfasser:in]; Yousef, Kaveh Pouran [Verfasser:in]; Duwe, Susanne [Verfasser:in]; Karsch, Katharina [Verfasser:in]; Grover, Sandeep [Verfasser:in]; Wählisch, Stephanie [Verfasser:in]; Obermeier, Patrick [Verfasser:in]; Tief, Franziska [Verfasser:in]; Mühlhans, Susann [Verfasser:in]; Seeber, Lea [Verfasser:in]; Kleist, Max von [Verfasser:in]; Schweiger, Brunhilde [Verfasser:in]; Rath, Barbara [Verfasser:in]

Quantitative Influenza Follow-Up Testing (QIFT) - A Novel Biomarker for the Monitoring of Disease Activity at the Point-of-Care

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  • Medientyp: E-Artikel
  • Titel: Quantitative Influenza Follow-Up Testing (QIFT) - A Novel Biomarker for the Monitoring of Disease Activity at the Point-of-Care
  • Beteiligte: Chen, Xi [Verfasser:in]; Yousef, Kaveh Pouran [Verfasser:in]; Duwe, Susanne [Verfasser:in]; Karsch, Katharina [Verfasser:in]; Grover, Sandeep [Verfasser:in]; Wählisch, Stephanie [Verfasser:in]; Obermeier, Patrick [Verfasser:in]; Tief, Franziska [Verfasser:in]; Mühlhans, Susann [Verfasser:in]; Seeber, Lea [Verfasser:in]; Kleist, Max von [Verfasser:in]; Schweiger, Brunhilde [Verfasser:in]; Rath, Barbara [Verfasser:in]
  • Erschienen: Freie Universität Berlin: Refubium (FU Berlin), 2014
  • Sprache: Englisch
  • DOI: https://doi.org/10.17169/refubium-21241; https://doi.org/10.1371/journal.pone.0092500
  • Entstehung:
  • Anmerkungen: Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
  • Beschreibung: Background Influenza infections induce considerable disease burden in young children. Biomarkers for the monitoring of disease activity at the point-of- care (POC) are currently lacking. Recent methodologies for fluorescence-based rapid testing have been developed to provide improved sensitivities with the initial diagnosis. The present study aims to explore the utility of second- generation rapid testing during longitudinal follow-up of influenza patients (Rapid Influenza Follow-up Testing = RIFT). Signal/control fluorescent readouts (Quantitative Influenza Follow-up Testing = QIFT) are evaluated as a potential biomarker for the monitoring of disease activity at the POC. Methods and Findings RIFT (SOFIA) and QIFT were performed at the POC and compared to blinded RT-PCR at the National Reference Centre for Influenza. From 10/2011-4/2013, a total of 2048 paediatric cases were studied prospectively; 273 cases were PCR-confirmed for influenza. During follow-up, RIFT results turned negative either prior to PCR (68%), or simultaneously (30%). The first negative RIFT occurred after a median of 8 days with a median virus load (VL) of 5.6×10∧3 copies/ml and cycle threshold of 37, with no evidence of viral rebound. Binning analysis revealed that QIFT differentiated accurately between patients with low, medium and high viral titres. QIFT increase/decrease showed 88% agreement (sensitivity = 52%, specificity = 95%) with VL increase/decrease, respectively. QIFT-based viral clearance estimates showed similar values compared to PCR-based estimates. Variations in viral clearance rates were lower in treated compared to untreated patients. The study was limited by use of non-invasive, semi-quantitative nasopharyngeal samples. VL measurements below the limit of detection could not be quantified reliably. Conclusions During follow-up, RIFT provides a first surrogate measure for influenza disease activity. A “switch” from positive to negative values may indicate a drop in viral load below a critical threshold, where rebound is no ...
  • Zugangsstatus: Freier Zugang
  • Rechte-/Nutzungshinweise: Namensnennung (CC BY)