Erschienen in:CLIB2021 2nd International Conference, Düsseldorf, Germany, 2011-04-06 - 2011-04-07
Sprache:
Englisch
Entstehung:
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Beschreibung:
Self-assembled proteolipid particles, termed 'nanodiscs', consist of approximately 150 lipid molecules arranged as a perfectly flat lipid bilayer. Two copies of an a polipoprotein A-I derived, recombinant Membrane scaffold protein (MSP) encircle the lipid patch and seal the hydrophobic edge of the bilayer from water. A single particle possesses a diameter of about 10 nm and a lipiddependent thickness of about 5 nm. The Diameter can be designed by the length of the MSP and the thickness is dependent from the choice of the lipid or lipid mixture. Up to date various membrane proteins have been successfully incorporated into nanodiscs in functional form. Recently, we presented the first solution NMR data of an integral membrane protein reconstituted in a nanodisc (J.Am. Chem. Soc. 2009, 131, 12060) demonstrating the high potential of the methodology not only for biophysical research, but also for application oriented purposes. The nanodisc system allows to prepare integral membrane Proteins completely detergent-free in solution with both sides of the lipid bilayer fully solvent (and substrate) accessible. This will allow preparation of enzymes and other proteins that are integral membrane proteins in conditions that are virtually indistinguishable from those of water soluble enzymes for biotechnological use.