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Beschreibung:
Oligosaccharides fulfil important purposes in biological processes, which exceeds by far the role of giving stability, storing energy and so on. The transfer of information by oligosaccharides plays e. g. in infections, fertilization, inflammations, folding of proteins or cell metastasis a crucial role. It is obvious that those structures can be used as drugs. Today, in most cases the cheap provision with sufficient amounts of defined oligosaccharides is still a problem. Besides the challenge of the synthesis itself, there is often the handicap of laborious and expensive column chromatography. In this thesis the approach of polyethylene glycol supported liquid phase synthesis of oligosaccharides was pursued. For purification $\textit{state of the art}$ ultrafiltration by ceramic membranes was used, allowing to work as well in organic as in aqueous media. One model [Abb. Polymer bound Galili trisaccaride as model system] system was the synthesis of $\omega$-methoxy polyethylene glycol bound so called Galili trisaccharide Gal$\alpha$-1,3-Gal$\beta$-1,4-GlcNAc. For providing the expensive activated sugar UDP-Galactose a regeneration system was used, meaning that the galactose unit of the target was finally supported by cheap sucrose. The present work comprises among others $\bullet$ membrane techniques for the retention of polymer supported oligosaccharides $\bullet$ the use of linkers for the reversible polymer linkage $\bullet$ the provision of $\beta$-1,4-galactosyltransferase $\bullet$ a chemical and an enzymatic polymer supported oligosaccharide synthesis $\bullet$ the establishment of a technique for the $\textit{direct}$ analysis of polymer supported molecules.