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Förster-Fromme, K. [VerfasserIn]; Höschle, B. [VerfasserIn]; Mack, C. [VerfasserIn]; Bott, M. [VerfasserIn]; Armbruster, W. [VerfasserIn]; Jendrossek, D. [VerfasserIn]

Identification of Genes and Proteins Necessary for Catabolism of Acyclic Terpenes and Leucine/Isovalerate in Pseudomonas aeruginosa

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  • Medientyp: E-Artikel
  • Titel: Identification of Genes and Proteins Necessary for Catabolism of Acyclic Terpenes and Leucine/Isovalerate in Pseudomonas aeruginosa
  • Beteiligte: Förster-Fromme, K. [VerfasserIn]; Höschle, B. [VerfasserIn]; Mack, C. [VerfasserIn]; Bott, M. [VerfasserIn]; Armbruster, W. [VerfasserIn]; Jendrossek, D. [VerfasserIn]
  • Erschienen: Soc., 2006
  • Erschienen in: Applied and environmental microbiology 72, 4819 - 4828 (2006). doi:10.1128/AEM.00853-06
  • Sprache: Englisch
  • DOI: https://doi.org/10.1128/AEM.00853-06
  • ISSN: 0099-2240
  • Schlagwörter: Monoterpenes: metabolism ; Western ; Electrophoresis ; Bacterial Proteins: metabolism ; Mutagenesis ; Two-Dimensional ; Monoterpenes ; Pseudomonas aeruginosa: genetics ; Leucine: metabolism ; Pentanoic Acids: metabolism ; citronellol ; Bacterial Proteins: genetics ; Insertional ; isovaleric acid ; Blotting ; DNA Transposable Elements ; Pseudomonas aeruginosa: metabolism ; Multigene Family ; Gel ; Carbon-Carbon Ligases: metabolism ; methylcrotonoyl-CoA carboxylase ; Terpenes: metabolism ; Bacterial Proteins ; [...]
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  • Beschreibung: Geranyl-coenzyme A (CoA)-carboxylase (GCase; AtuC/AtuF) and methylcrotonyl-CoA-carboxylase (MCase; LiuB/LiuD) are characteristic enzymes of the catabolic pathway of acyclic terpenes (citronellol and geraniol) and of saturated methyl-branched compounds, such as leucine or isovalerate, respectively. Proteins encoded by two gene clusters (atuABCDEFGH and liuRABCDE) of Pseudomonas aeruginosa PAO1 were essential for acyclic terpene utilization (Atu) and for leucine and isovalerate utilization (Liu), respectively, as revealed by phenotype analysis of 10 insertion mutants, two-dimensional gel electrophoresis, determination of GCase and MCase activities, and Western blot analysis of wild-type and mutant strains. Analysis of the genome sequences of other pseudomonads (P. putida KT2440 and P. fluorescens Pf-5) revealed candidate genes for Liu proteins for both species and candidate genes for Atu proteins in P. fluorescens. This result concurred with the finding that P. fluorescens, but not P. putida, could grow on acyclic terpenes (citronellol and citronellate), while both species were able to utilize leucine and isovalerate. A regulatory gene, atuR, was identified upstream of atuABCDEFGH and negatively regulated expression of the atu gene cluster.
  • Zugangsstatus: Freier Zugang