• Medientyp: E-Artikel
  • Titel: Surface sticking and lateral diffusion of lipids in supported bilayers
  • Beteiligte: Dertinger, T. [Verfasser:in]; von der Hocht, I. [Verfasser:in]; Benda, A. [Verfasser:in]; Hof, M. [Verfasser:in]; Enderlein, J. [Verfasser:in]
  • Erschienen: ACS Publ., 2006
  • Erschienen in: Langmuir 22, 9339 - 9344 (2006). doi:10.1021/la061389s
  • Sprache: Englisch
  • DOI: https://doi.org/10.1021/la061389s
  • ISSN: 0743-7463
  • Schlagwörter: Surface Properties ; Diffusion ; Lipid Bilayers: chemistry ; Lipid Bilayers
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  • Beschreibung: The diffusion of fluorescently labeled lipids in supported bilayers is studied using two different methods: Z-scan fluorescence correlation spectroscopy (z-scan FCS) and two-focus fluorescence correlation spectroscopy (2f-FCS). It is found that the data can be fitted consistently only when taking into account partial sticking of the labeled lipids to the supporting glass surface. A kinetic reaction-diffusion model is developed and applied to the data. We find a very slow sticking rate which, however, when neglected, leads to strongly varying estimates of the free diffusion coefficient. The study reveals a strong sensitivity of FCS on even slight binding/unbinding kinetics of the labeled molecules, which has significance for related diffusion measurements in cellular lipid membranes.
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