Oberleitner, Linda
[VerfasserIn];
Poschmann, Gereon
[VerfasserIn];
Macorano, Luis
[VerfasserIn];
Schott-Verdugo, Stephan
[VerfasserIn];
Gohlke, Holger
[VerfasserIn];
Stühler, Kai
[VerfasserIn];
Nowack, Eva C. M.
[VerfasserIn]
The Puzzle of Metabolite Exchange and Identification of Putative Octotrico Peptide Repeat Expression Regulators in the Nascent Photosynthetic Organelles of Paulinella chromatophora
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Medientyp:
E-Artikel
Titel:
The Puzzle of Metabolite Exchange and Identification of Putative Octotrico Peptide Repeat Expression Regulators in the Nascent Photosynthetic Organelles of Paulinella chromatophora
Beteiligte:
Oberleitner, Linda
[VerfasserIn];
Poschmann, Gereon
[VerfasserIn];
Macorano, Luis
[VerfasserIn];
Schott-Verdugo, Stephan
[VerfasserIn];
Gohlke, Holger
[VerfasserIn];
Stühler, Kai
[VerfasserIn];
Nowack, Eva C. M.
[VerfasserIn]
Erschienen:
Frontiers Media, 2020
Erschienen in:Frontiers in microbiology 11, 607182 (2020). doi:10.3389/fmicb.2020.607182
Sprache:
Englisch
DOI:
https://doi.org/10.3389/fmicb.2020.607182
ISSN:
1664-302X
Entstehung:
Anmerkungen:
Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
Beschreibung:
The endosymbiotic acquisition of mitochondria and plastids more than one billion years ago was central for the evolution of eukaryotic life. However, owing to their ancient origin, these organelles provide only limited insights into the initial stages of organellogenesis. The cercozoan amoeba Paulinella chromatophora contains photosynthetic organelles—termed chromatophores—that evolved from a cyanobacterium ∼100 million years ago, independently from plastids in plants and algae. Despite the more recent origin of the chromatophore, it shows tight integration into the host cell. It imports hundreds of nucleus-encoded proteins, and diverse metabolites are continuously exchanged across the two chromatophore envelope membranes. However, the limited set of chromatophore-encoded solute transporters appears insufficient for supporting metabolic connectivity or protein import. Furthermore, chromatophore-localized biosynthetic pathways as well as multiprotein complexes include proteins of dual genetic origin, suggesting that mechanisms evolved that coordinate gene expression levels between chromatophore and nucleus. These findings imply that similar to the situation in mitochondria and plastids, also in P. chromatophora nuclear factors evolved that control metabolite exchange and gene expression in the chromatophore. Here we show by mass spectrometric analyses of enriched insoluble protein fractions that, unexpectedly, nucleus-encoded transporters are not inserted into the chromatophore inner envelope membrane. Thus, despite the apparent maintenance of its barrier function, canonical metabolite transporters are missing in this membrane. Instead we identified several expanded groups of short chromatophore-targeted orphan proteins. Members of one of these groups are characterized by a single transmembrane helix, and others contain amphipathic helices. We hypothesize that these proteins are involved in modulating membrane permeability. Thus, the mechanism generating metabolic connectivity of the chromatophore fundamentally ...