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Couto, Joao [VerfasserIn]; Musall, Simon [VerfasserIn]; Churchland, 9and Anne K. [VerfasserIn]; Sun, Xiaonan R. [VerfasserIn]; Khanal, Anup [VerfasserIn]; Gluf, Steven [VerfasserIn]; Saxena, Shreya [VerfasserIn]; Kinsella, Ian [VerfasserIn]; Abe, Taiga [VerfasserIn]; Cunningham, John P. [VerfasserIn]; Paninski, Liam [VerfasserIn]

Chronic, cortex-wide imaging of specific cell populations during behavior

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  • Medientyp: E-Artikel
  • Titel: Chronic, cortex-wide imaging of specific cell populations during behavior
  • Beteiligte: Couto, Joao [VerfasserIn]; Musall, Simon [VerfasserIn]; Churchland, 9and Anne K. [VerfasserIn]; Sun, Xiaonan R. [VerfasserIn]; Khanal, Anup [VerfasserIn]; Gluf, Steven [VerfasserIn]; Saxena, Shreya [VerfasserIn]; Kinsella, Ian [VerfasserIn]; Abe, Taiga [VerfasserIn]; Cunningham, John P. [VerfasserIn]; Paninski, Liam [VerfasserIn]
  • Erschienen: Nature Publishing Group, 2021
  • Erschienen in: Nature protocols 16, 3241–3263 (2021). doi:10.1038/s41596-021-00527-z
  • Sprache: Englisch
  • DOI: https://doi.org/10.1038/s41596-021-00527-z
  • ISSN: 1754-2189; 1750-2799
  • Entstehung:
  • Anmerkungen: Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
  • Beschreibung: Measurements of neuronal activity across brain areas are important for understanding the neural correlates of cognitive and motor processes such as attention, decision-making and action selection. However, techniques that allow cellular resolution measurements are expensive and require a high degree of technical expertise, which limits their broad use. Wide-field imaging of genetically encoded indicators is a high-throughput, cost-effective and flexible approach to measure activity of specific cell populations with high temporal resolution and a cortex-wide field of view. Here we outline our protocol for assembling a wide-field macroscope setup, performing surgery to prepare the intact skull and imaging neural activity chronically in behaving, transgenic mice. Further, we highlight a processing pipeline that leverages novel, cloud-based methods to analyze large-scale imaging datasets. The protocol targets laboratories that are seeking to build macroscopes, optimize surgical procedures for long-term chronic imaging and/or analyze cortex-wide neuronal recordings. The entire protocol, including steps for assembly and calibration of the macroscope, surgical preparation, imaging and data analysis, requires a total of 8 h. It is designed to be accessible to laboratories with limited expertise in imaging methods or interest in high-throughput imaging during behavior.
  • Zugangsstatus: Freier Zugang