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Barthel, Tatjana [VerfasserIn]; Huschmann, Franziska U. [VerfasserIn]; Wallacher, Dirk [VerfasserIn]; Feiler, Christian G. [VerfasserIn]; Klebe, Gerhard [VerfasserIn]; Weiss, Manfred S. [VerfasserIn]; Wollenhaupt, Jan [VerfasserIn]; 1Helmholtz-Zentrum Berlin, Macromolecular Crystallography, Albert-Einstein-Straße 15, 12489Berlin, Germany [VerfasserIn]; 4Helmholtz-Zentrum Berlin, Department Sample Environment, Hahn-Meitner-Platz 1, 14109Berlin, Germany [VerfasserIn]; 3Philipps-Universität Marburg, Institute of Pharmaceutical Chemistry, Drug Design Group, Marbacher Weg 6, 35032Marburg, Germany [VerfasserIn]

Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates

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  • Medientyp: E-Artikel
  • Titel: Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates
  • Beteiligte: Barthel, Tatjana [VerfasserIn]; Huschmann, Franziska U. [VerfasserIn]; Wallacher, Dirk [VerfasserIn]; Feiler, Christian G. [VerfasserIn]; Klebe, Gerhard [VerfasserIn]; Weiss, Manfred S. [VerfasserIn]; Wollenhaupt, Jan [VerfasserIn]; 1Helmholtz-Zentrum Berlin, Macromolecular Crystallography, Albert-Einstein-Straße 15, 12489Berlin, Germany [VerfasserIn]; 4Helmholtz-Zentrum Berlin, Department Sample Environment, Hahn-Meitner-Platz 1, 14109Berlin, Germany [VerfasserIn]; 3Philipps-Universität Marburg, Institute of Pharmaceutical Chemistry, Drug Design Group, Marbacher Weg 6, 35032Marburg, Germany [VerfasserIn]
  • Erschienen: International Union of Crystallography; 5 Abbey Square, Chester, Cheshire CH1 2HU, England, 2021-01-29
  • Sprache: Englisch
  • DOI: https://doi.org/10.23689/fidgeo-4071
  • Schlagwörter: evaporation reduction ; crystal handling ; crystal harvesting ; crystallographic fragment screening
  • Entstehung:
  • Anmerkungen: Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
  • Beschreibung: In the past two decades, most of the steps in a macromolecular crystallography experiment have undergone tremendous development with respect to speed, feasibility and increase of throughput. The part of the experimental workflow that is still a bottleneck, despite significant efforts, involves the manipulation and harvesting of the crystals for the diffraction experiment. Here, a novel low‐cost device is presented that functions as a cover for 96‐well crystallization plates. This device enables access to the individual experiments one at a time by its movable parts, while minimizing evaporation of all other experiments of the plate. In initial tests, drops of many typically used crystallization cocktails could be successfully protected for up to 6 h. Therefore, the manipulation and harvesting of crystals is straightforward for the experimenter, enabling significantly higher throughput. This is useful for many macromolecular crystallography experiments, especially multi‐crystal screening campaigns. ; A simple and low‐cost device has been developed to minimize evaporation in microtiter plates for easy crystal handling and harvesting. image
  • Zugangsstatus: Freier Zugang