> Detailanzeige

Meurer, Marita [Verfasser:in]; de Buhr, Nicole [Verfasser:in]; Unger, Linn Meret [Verfasser:in]; Bonilla, Marta C. [Verfasser:in]; Seele, Jana [Verfasser:in]; Nau, Roland [Verfasser:in]; Baums, Christoph G. [Verfasser:in]; Gutsmann, Thomas [Verfasser:in]; Schwarz, Stefan [Verfasser:in]; von Köckritz-Blickwede, Maren [Verfasser:in]

Comparing Cathelicidin Susceptibility of the Meningitis Pathogens Streptococcus suis and Escherichia coli in Culture Medium in Contrast to Porcine or Human Cerebrospinal Fluid

Literatur-
verwaltung

Zur
Merkliste

Lösche von
Merkliste

Per Whatsapp teilen

Schließen

Merkliste



Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
  • Medientyp: E-Artikel
  • Titel: Comparing Cathelicidin Susceptibility of the Meningitis Pathogens Streptococcus suis and Escherichia coli in Culture Medium in Contrast to Porcine or Human Cerebrospinal Fluid
  • Beteiligte: Meurer, Marita [Verfasser:in]; de Buhr, Nicole [Verfasser:in]; Unger, Linn Meret [Verfasser:in]; Bonilla, Marta C. [Verfasser:in]; Seele, Jana [Verfasser:in]; Nau, Roland [Verfasser:in]; Baums, Christoph G. [Verfasser:in]; Gutsmann, Thomas [Verfasser:in]; Schwarz, Stefan [Verfasser:in]; von Köckritz-Blickwede, Maren [Verfasser:in]
  • Erschienen: Lausanne: Frontiers Research Foundation, [2023]
  • Erschienen in: Frontiers in microbiology ; 10, (2019)
  • Sprache: Englisch
  • Schlagwörter: Streptococcus suis ; LL-37 ; AMP ; PR-39 ; Escherichia coli ; MIC ; cerebrospinal fluid (CSF)
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: Host defense peptides or antimicrobial peptides (AMPs), e.g., cathelicidins, haverecently been discussed as a potential new treatment option against bacterial infections.To test the efficacy of AMPs, standardized methods that closely mimic the physiologicalconditions at the site of infection are still needed. The aim of our study was totest the meningitis-causing bacteria Streptococcus suis and Escherichia coli for theirsusceptibility to cathelicidins in culture medium versus cerebrospinal fluid (CSF).Susceptibility testing was performed in analogy to the broth microdilution methoddescribed by the Clinical and Laboratory Standard Institute (CLSI) to determine minimuminhibitory concentrations (MICs) of antimicrobial agents. MICs were determined usingcation-adjusted Mueller–Hinton broth (CA-MHB), lysogeny broth (LB), Roswell ParkMemorial Institute medium (RPMI) or Dulbecco’s Modified Eagle’s Medium (DMEM)(the latter two supplemented with 5% CA-MHB or blood) and compared with MICsobtained in porcine or human CSF. Our data showed that MICs obtained in CA-MHBas recommended by CLSI do not reflect the MICs obtained in the physiological bodyfluid CSF. However, the MICs of clinical isolates of S. suis tested in RPMI mediumsupplemented with CA-MHB, were similar to those of the same strains tested in CSF.In contrast, the MICs in the human CSF for the tested E. coli K1 strain were highercompared to the RPMI medium and showed even higher values than in CA-MHB. Thishighlights the need for susceptibility testing of AMPs in a medium that closely mimicsthe clinically relevant conditions.
  • Zugangsstatus: Freier Zugang
  • Rechte-/Nutzungshinweise: Namensnennung (CC BY)