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Medientyp:
E-Artikel
Titel:
Intermediate filaments ensure resiliency of single carcinoma cells, while active contractility of the actin cortex determines their invasive potential
Beteiligte:
Ficorella, Carlotta
[VerfasserIn];
Eichholz, Hannah Marie
[VerfasserIn];
Sala, Federico
[VerfasserIn];
Vázquez, Rebeca Martínez
[VerfasserIn];
Osellame, Roberto
[VerfasserIn];
Käs, Josef A.
[VerfasserIn]
Beschreibung:
During the epithelial-to-mesenchymal transition, the intracellular cytoskeleton undergoes severereorganization which allows epithelial cells to transition into a motile mesenchymal phenotype.Among the different cytoskeletal elements, the intermediate filaments keratin (in epithelial cells)and vimentin (in mesenchymal cells) have been demonstrated to be useful and reliable histologicalmarkers. In this study, we assess the potential invasiveness of six human breast carcinoma cell linesand two mouse fibroblasts cells lines through single cell migration assays in confinement. We findthat the keratin and vimentin networks behave mechanically the same when cells crawl throughnarrow channels and that vimentin protein expression does not strongly correlate to single cellsinvasiveness. Instead, we find that what determines successful migration through confining spacesis the ability of cells to mechanically switch from a substrate-dependent stress fibers basedcontractility to a substrate-independent cortical contractility, which is not linked to their tumorphenotype.