Beschreibung:
<jats:title>Abstract</jats:title><jats:p>The human disease hyperekplexia is characterized by excessive startle reactions to auditory and cutaneous stimuli. In its familial form, hyperekplexia has been associated with both dominant and recessive mutations of the GLRA1 gene encoding the glycine receptor α<jats:sub>1</jats:sub> subunit (GlyRα1), which mediates inhibitory transmission in the spinal cord and brainstem. Here we have examined the functional consequences of two amino acid substitutions found in a compound heterozygous family, R252H and R392H, to investigate the mechanisms determining this inheritance pattern. When expressed in <jats:italic>Xenopus laevis</jats:italic> oocytes, both mutations were non‐functional. Neither mutant affected the electrophysiological properties of wild type GlyRα1 when co‐expressed. We introduced a green fluorescent protein tag to mutant subunits and found that both mutant proteins were detectable. Evidence that subcellular localization differed from wild type was significant for one of the mutants. Thus, an effective loss of functional GlyRα1‐mediated current underlies hyperekplexia in this family, whereas a partial loss is asymptomatic.</jats:p>