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Medientyp:
E-Artikel
Titel:
Characterization of LRP, a leucine‐rich repeat (LRR) protein from tomato plants that is processed during pathogenesis
Beteiligte:
Tornero, Pablo;
Mayda, Esther;
Gómez, María Dolores;
Cañas, Luis;
Conejero, Vicente;
Vera, Pablo
Erschienen:
Wiley, 1996
Erschienen in:The Plant Journal
Sprache:
Englisch
DOI:
10.1046/j.1365-313x.1996.10020315.x
ISSN:
0960-7412;
1365-313X
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>This paper describes the isolation and characterization of <jats:italic>LRP</jats:italic>, a new gene from tomato plants. The deduced amino acid sequence showed that the encoded protein is enriched in leucine, and contains interesting structural motifs. <jats:italic>LRP</jats:italic> contains four tandem repeats of a canonical 24 amino acid leucine‐rich repeat (LRR) sequence present in different proteins that mediates molecular recognition and/or interaction processes. Genomic organization and intron‐exon arrangement of <jats:italic>LRP</jats:italic> favor the hypothesis that the LRR domains present in <jats:italic>LRP</jats:italic> evolved by exon duplication and shuffling. <jats:italic>LRP</jats:italic> expression analysis and immunohisto‐chemical localization studies of the encoded protein indicate that the gene is under developmental regulation exhibiting tissue‐specificity, particularly in certain cell types of the stele, like phloem fibers, parenchyma cells of the protoxylem, and in the cell files that constitute the rays of the secondary xylem. It is shown that this gene is upregulated in diseased tomato plants infected with citrus exocortis viroid. However, in this pathogenic context, LRP is processed proteolytically to a lower molecular weight form by a host‐induced extracellular protease. The structural characteristics of LRP, its spatio‐temporal pattern of expression, and its post‐translational processing during pathogenesis, suggest this protein as a candidate molecule that may mediate recognition and interaction events taking place in the plant extracellular matrix under normal and/or pathogenesis‐related conditions.</jats:p>