• Medientyp: E-Artikel
  • Titel: Linearly modulated multi-focal diffractive lens for multi-sheet excitation of flow driven samples in a light-sheet fluorescence microscope
  • Beteiligte: Hofmann, Meike; Gharbi Ghebjagh, Shima; Feng, Yuchao; Fan, Chao; Lemke, Karen; Sinzinger, Stefan
  • Erschienen: EDP Sciences, 2023
  • Erschienen in: Journal of the European Optical Society-Rapid Publications, 19 (2023) 1, Seite 26
  • Sprache: Ohne Angabe
  • DOI: 10.1051/jeos/2023022
  • ISSN: 1990-2573
  • Schlagwörter: Atomic and Molecular Physics, and Optics
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  • Anmerkungen:
  • Beschreibung: Light sheet fluorescence microscope with single light sheet illumination enables rapid 3D imaging of living cells. In this paper we show the design, fabrication and characterization of a diffractive optical element producing several light sheets along a 45° inclined tube. The element, which is based on a multi-focal diffractive lens and a linear grating, generates five thin light sheets with equal intensities when combined with a refractive cylindrical lens. The generated uniform light sheets can be applied for the scanning of samples in tubes enabling flow-driven 3-dimensional imaging.
  • Zugangsstatus: Freier Zugang