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Medientyp:
E-Artikel
Titel:
High-power near-concentric Fabry–Perot cavity for phase contrast electron microscopy
Beteiligte:
Turnbaugh, Carter;
Axelrod, Jeremy J.;
Campbell, Sara L.;
Dioquino, Jeske Y.;
Petrov, Petar N.;
Remis, Jonathan;
Schwartz, Osip;
Yu, Zanlin;
Cheng, Yifan;
Glaeser, Robert M.;
Mueller, Holger
Erschienen:
AIP Publishing, 2021
Erschienen in:
Review of Scientific Instruments, 92 (2021) 5
Sprache:
Englisch
DOI:
10.1063/5.0045496
ISSN:
0034-6748;
1089-7623
Entstehung:
Anmerkungen:
Beschreibung:
Transmission electron microscopy (TEM) of vitrified biological macromolecules (cryo-EM) is limited by the weak phase contrast signal that is available from such samples. Using a phase plate would thus substantially improve the signal-to-noise ratio. We have previously demonstrated the use of a high-power Fabry–Perot cavity as a phase plate for TEM. We now report improvements to our laser cavity that allow us to achieve record continuous wave intensities of over 450 GW/cm2, sufficient to produce the optimal 90° phase shift for 300 keV electrons. In addition, we have performed the first cryo-EM reconstruction using a laser phase plate, demonstrating that the stability of this laser phase plate is sufficient for use during standard cryo-EM data collection.