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Medientyp:
E-Artikel
Titel:
A direct role of SRY and SOX proteins in pre-mRNA splicing
Beteiligte:
Ohe, Kenji;
Lalli, Enzo;
Sassone-Corsi, Paolo
Erschienen:
Proceedings of the National Academy of Sciences, 2002
Erschienen in:
Proceedings of the National Academy of Sciences, 99 (2002) 3, Seite 1146-1151
Sprache:
Englisch
DOI:
10.1073/pnas.022645899
ISSN:
0027-8424;
1091-6490
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>
The mammalian testis determining factor SRY and its related Sox factors are critical developmental regulators. They share significant similarity in their high mobility group (HMG) domain and display discrete patterns of tissue-specific expression. Here we show that SRY and the Sox protein SOX6 colocalize with splicing factors in the nucleus and are dynamically redistributed following the blockage of splicing in living cells. Anti-SOX6 antibodies supershift the spliceosomal complex from assembled splicing reactions and inhibit splicing
<jats:italic>in vitro</jats:italic>
of multiple pre-mRNA substrates. Most importantly, SOX6-depleted nuclear extracts have impaired splicing activity, which is efficiently restored by addition of the recombinant SOX6 HMG domain and also by recombinant SRY and the SOX9 HMG domain. These results reveal an unexpected biological function of the
<jats:italic>SRY</jats:italic>
,
<jats:italic>SOX6</jats:italic>
, and
<jats:italic>SOX9</jats:italic>
gene products and provide a functional link to the biochemical mechanisms operating in mammalian sex determination and in other developmental processes regulated by
<jats:italic>Sox</jats:italic>
genes.
</jats:p>