Chang, Dong-Eun;
Smalley, Darren J.;
Tucker, Don L.;
Leatham, Mary P.;
Norris, Wendy E.;
Stevenson, Sarah J.;
Anderson, April B.;
Grissom, Joe E.;
Laux, David C.;
Cohen, Paul S.;
Conway, Tyrrell
Carbon nutrition of Escherichia coli in the mouse intestine
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Medientyp:
E-Artikel
Titel:
Carbon nutrition of Escherichia coli in the mouse intestine
Beteiligte:
Chang, Dong-Eun;
Smalley, Darren J.;
Tucker, Don L.;
Leatham, Mary P.;
Norris, Wendy E.;
Stevenson, Sarah J.;
Anderson, April B.;
Grissom, Joe E.;
Laux, David C.;
Cohen, Paul S.;
Conway, Tyrrell
Erschienen:
Proceedings of the National Academy of Sciences, 2004
Erschienen in:Proceedings of the National Academy of Sciences
Sprache:
Englisch
DOI:
10.1073/pnas.0307888101
ISSN:
0027-8424;
1091-6490
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>
Whole-genome expression profiling revealed
<jats:italic>Escherichia coli</jats:italic>
MG1655 genes induced by growth on mucus, conditions designed to mimic nutrient availability in the mammalian intestine. Most were nutritional genes corresponding to catabolic pathways for nutrients found in mucus. We knocked out several pathways and tested the relative fitness of the mutants for colonization of the mouse intestine in competition with their wild-type parent. We found that only mutations in sugar pathways affected colonization, not phospholipid and amino acid catabolism, not gluconeogenesis, not the tricarboxylic acid cycle, and not the pentose phosphate pathway. Gluconate appeared to be a major carbon source used by
<jats:italic>E. coli</jats:italic>
MG1655 to colonize, having an impact on both the initiation and maintenance stages.
<jats:italic>N</jats:italic>
-acetylglucosamine and
<jats:italic>N</jats:italic>
-acetylneuraminic acid appeared to be involved in initiation, but not maintenance. Glucuronate, mannose, fucose, and ribose appeared to be involved in maintenance, but not initiation. The
<jats:italic>in vitro</jats:italic>
order of preference for these seven sugars paralleled the relative impact of the corresponding metabolic lesions on colonization: gluconate >
<jats:italic>N</jats:italic>
-acetylglucosamine >
<jats:italic>N-</jats:italic>
acetylneuraminic acid = glucuronate > mannose > fucose > ribose. The results of this systematic analysis of nutrients used by
<jats:italic>E. coli</jats:italic>
MG1655 to colonize the mouse intestine are intriguing in light of the nutrient-niche hypothesis, which states that the ecological niches within the intestine are defined by nutrient availability. Because humans are presumably colonized with different commensal strains, differences in nutrient availability may provide an open niche for infecting
<jats:italic>E. coli</jats:italic>
pathogens in some individuals and a barrier to infection in others.
</jats:p>