Erschienen:
Proceedings of the National Academy of Sciences, 2018
Erschienen in:Proceedings of the National Academy of Sciences
Sprache:
Englisch
DOI:
10.1073/pnas.1720862115
ISSN:
0027-8424;
1091-6490
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Significance</jats:title>
<jats:p>Three-dimensional histology of soft-tissue samples has proven to provide crucial benefits for the understanding of tissue structure. Nevertheless, the low attenuation of soft tissue has impaired the use of computed tomography (CT) as a tool for the nondestructive and 3D visualization of external and internal structural details. We present a cytoplasm-specific staining method tailored for X-ray CT that enables a routine and efficient 3D volume screening at high resolutions. Our technique is fully compatible with conventional histology and allows further histological investigations, as demonstrated for a mouse kidney. The comparative analysis of our CT data with conventional 2D histology highlights the future applicability of the cytoplasm-specific X-ray staining method for modern histological and histopathological investigations using laboratory X-ray CT devices.</jats:p>