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Jones, Adam C.; Monroe, Emily A.; Podell, Sheila; Hess, Wolfgang R.; Klages, Sven; Esquenazi, Eduardo; Niessen, Sherry; Hoover, Heather; Rothmann, Michael; Lasken, Roger S.; Yates, John R.; Reinhardt, Richard; Kube, Michael; Burkart, Michael D.; Allen, Eric E.; Dorrestein, Pieter C.; Gerwick, William H.; Gerwick, Lena

Genomic insights into the physiology and ecology of the marine filamentous cyanobacterium Lyngbya majuscula

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  • Medientyp: E-Artikel
  • Titel: Genomic insights into the physiology and ecology of the marine filamentous cyanobacterium Lyngbya majuscula
  • Beteiligte: Jones, Adam C.; Monroe, Emily A.; Podell, Sheila; Hess, Wolfgang R.; Klages, Sven; Esquenazi, Eduardo; Niessen, Sherry; Hoover, Heather; Rothmann, Michael; Lasken, Roger S.; Yates, John R.; Reinhardt, Richard; Kube, Michael; Burkart, Michael D.; Allen, Eric E.; Dorrestein, Pieter C.; Gerwick, William H.; Gerwick, Lena
  • Erschienen: Proceedings of the National Academy of Sciences, 2011
  • Erschienen in: Proceedings of the National Academy of Sciences
  • Sprache: Englisch
  • DOI: 10.1073/pnas.1101137108
  • ISSN: 0027-8424; 1091-6490
  • Entstehung:
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  • Beschreibung: <jats:p> Filamentous cyanobacteria of the genus <jats:italic>Lyngbya</jats:italic> are important contributors to coral reef ecosystems, occasionally forming dominant cover and impacting the health of many other co-occurring organisms. Moreover, they are extraordinarily rich sources of bioactive secondary metabolites, with 35% of all reported cyanobacterial natural products deriving from this single pantropical genus. However, the true natural product potential and life strategies of <jats:italic>Lyngbya</jats:italic> strains are poorly understood because of phylogenetic ambiguity, lack of genomic information, and their close associations with heterotrophic bacteria and other cyanobacteria. To gauge the natural product potential of <jats:italic>Lyngbya</jats:italic> and gain insights into potential microbial interactions, we sequenced the genome of <jats:italic>Lyngbya majuscula</jats:italic> 3L, a Caribbean strain that produces the tubulin polymerization inhibitor curacin A and the molluscicide barbamide, using a combination of Sanger and 454 sequencing approaches. Whereas ∼293,000 nucleotides of the draft genome are putatively dedicated to secondary metabolism, this is far too few to encode a large suite of <jats:italic>Lyngbya</jats:italic> metabolites, suggesting <jats:italic>Lyngbya</jats:italic> metabolites are strain specific and may be useful in species delineation. Our analysis revealed a complex gene regulatory network, including a large number of sigma factors and other regulatory proteins, indicating an enhanced ability for environmental adaptation or microbial associations. Although <jats:italic>Lyngbya</jats:italic> species are reported to fix nitrogen, nitrogenase genes were not found in the genome or by PCR of genomic DNA. Subsequent growth experiments confirmed that <jats:italic>L. majuscula</jats:italic> 3L is unable to fix atmospheric nitrogen. These unanticipated life history characteristics challenge current views of the genus <jats:italic>Lyngbya</jats:italic> . </jats:p>
  • Zugangsstatus: Freier Zugang