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Medientyp:
E-Artikel
Titel:
CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing
Beteiligte:
Kuscu, Canan;
Zaratiegui, Mikel;
Kim, Hyun Soo;
Wah, David A.;
Martienssen, Robert A.;
Schalch, Thomas;
Joshua-Tor, Leemor
Erschienen:
Proceedings of the National Academy of Sciences, 2014
Erschienen in:Proceedings of the National Academy of Sciences
Sprache:
Englisch
DOI:
10.1073/pnas.1313096111
ISSN:
0027-8424;
1091-6490
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Significance</jats:title>
<jats:p>
The CLRC complex is essential for heterochromatin formation in the yeast
<jats:italic>Schizosaccharomyces pombe.</jats:italic>
Its well-known role in placing methyl marks on histone H3 lysine 9 at heterochromatic loci is attributed to one of its components, cryptic loci regulator 4. However, it also contains an E3 ubiquitin ligase, a less understood activity of this complex. Here, we describe the organization of this seven-component complex and determine the crystal structure of delocalization of Swi6 1 (Dos1), a key subunit involved in targeting CLRC. We identify Dos2 as the central component of the complex and point of contact with Stc1, which bridges CLRC to the RNAi-induced transcriptional silencing complex, and show that heterochromatin formation is dependent on an exposed surface of Dos1. These results provide an unprecedented, high-resolution functional annotation of CLRC.
</jats:p>