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Vrana, Kent E.; Hipp, Jason D.; Goss, Ashley M.; McCool, Brian A.; Riddle, David R.; Walker, Stephen J.; Wettstein, Peter J.; Studer, Lorenz P.; Tabar, Viviane; Cunniff, Kerrianne; Chapman, Karen; Vilner, Lucy; West, Michael D.; Grant, Kathleen A.; Cibelli, Jose B.

Nonhuman primate parthenogenetic stem cells

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  • Medientyp: E-Artikel
  • Titel: Nonhuman primate parthenogenetic stem cells
  • Beteiligte: Vrana, Kent E.; Hipp, Jason D.; Goss, Ashley M.; McCool, Brian A.; Riddle, David R.; Walker, Stephen J.; Wettstein, Peter J.; Studer, Lorenz P.; Tabar, Viviane; Cunniff, Kerrianne; Chapman, Karen; Vilner, Lucy; West, Michael D.; Grant, Kathleen A.; Cibelli, Jose B.
  • Erschienen: Proceedings of the National Academy of Sciences, 2003
  • Erschienen in: Proceedings of the National Academy of Sciences
  • Sprache: Englisch
  • DOI: 10.1073/pnas.2034195100
  • ISSN: 0027-8424; 1091-6490
  • Schlagwörter: Multidisciplinary
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:p>Parthenogenesis is the biological phenomenon by which embryonic development is initiated without male contribution. Whereas parthenogenesis is a common mode of reproduction in lower organisms, the mammalian parthenote fails to produce a successful pregnancy. We herein describe<jats:italic>in vitro</jats:italic>parthenogenetic development of monkey (<jats:italic>Macaca fascicularis</jats:italic>) eggs to the blastocyst stage, and their use to create a pluripotent line of stem cells. These monkey stem cells (Cyno-1 cells) are positive for telomerase activity and are immunoreactive for alkaline phosphatase, octamer-binding transcription factor 4 (Oct-4), stage-specific embryonic antigen 4 (SSEA-4), tumor rejection antigen 1-60 (TRA 1-60), and tumor rejection antigen 1-81 (TRA 1-81) (traditional markers of human embryonic stem cells). They have a normal chromosome karyotype (40 + 2) and can be maintained<jats:italic>in vitro</jats:italic>in an undifferentiated state for extended periods of time. Cyno-1 cells can be differentiated<jats:italic>in vitro</jats:italic>into dopaminergic and serotonergic neurons, contractile cardiomyocyte-like cells, smooth muscle, ciliated epithelia, and adipocytes. When Cyno-1 cells were injected into severe combined immunodeficient mice, teratomas with derivatives from all three embryonic germ layers were obtained. When grown on fibronectin/laminin-coated plates and in neural progenitor medium, Cyno-1 cells assume a neural precursor phenotype (immunoreactive for nestin). However, these cells remain proliferative and express no functional ion channels. When transferred to differentiation conditions, the nestin-positive precursors assume neuronal and epithelial morphologies. Over time, these cells acquire electrophysiological characteristics of functional neurons (appearance of tetrodotoxin-sensitive, voltage-dependent sodium channels). These results suggest that stem cells derived from the parthenogenetically activated nonhuman primate egg provide a potential source for autologous cell therapy in the female and bypass the need for creating a competent embryo.</jats:p>
  • Zugangsstatus: Freier Zugang