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Medientyp:
E-Artikel
Titel:
Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells
Beteiligte:
Luo, Guangbin;
Ivics, Zoltán;
Izsvák, Zsuzsanna;
Bradley, Allan
Erschienen:
Proceedings of the National Academy of Sciences, 1998
Erschienen in:
Proceedings of the National Academy of Sciences, 95 (1998) 18, Seite 10769-10773
Sprache:
Englisch
DOI:
10.1073/pnas.95.18.10769
ISSN:
0027-8424;
1091-6490
Entstehung:
Anmerkungen:
Beschreibung:
Mouse has become an increasingly important organism for modeling human diseases and for determining gene function in a mammalian context. Unfortunately, transposon-tagged mutagenesis, one of the most valuable tools for functional genomics, still is not available in this organism. On the other hand, it has long been speculated that members of the Tc1/mariner -like elements may be less dependent on host factors and, hence, can be introduced into heterologous organisms. However, this prediction has not been realized in mice. We report here the chromosomal transposition of the Sleeping Beauty ( SB ) element in mouse embryonic stem cells, providing evidence that it can be used as an in vivo mutagen in mice.