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Medientyp:
E-Artikel
Titel:
5-Lipoxygenase expression in Langerhans cells of normal human epidermis
Beteiligte:
Spanbroek, Rainer;
Stark, Hans-Jürgen;
Janßen-Timmen, Uwe;
Kraft, Stefan;
Hildner, Markus;
Andl, Thomas;
Bosch, Franz-Xaver;
Fusenig, Norbert E.;
Bieber, Thomas;
Rådmark, Olof;
Samuelsson, Bengt;
Habenicht, Andreas J. R.
Erschienen:
Proceedings of the National Academy of Sciences, 1998
Erschienen in:Proceedings of the National Academy of Sciences
Sprache:
Englisch
DOI:
10.1073/pnas.95.2.663
ISSN:
0027-8424;
1091-6490
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>
We studied expression of the 5-lipoxygenase (5-LO) pathway in normal human skin.
<jats:italic>In situ</jats:italic>
hybridization revealed a 5-LO mRNA-containing epidermal cell (EC) population that was predominantly located in the midportion of the spinous layer, in outer hair root sheaths, and in the epithelial compartment of sebaceous glands. Examination of skin specimens by immunohistochemistry and of primary ECs by flow cytometry mapped the 5-LO protein exclusively to Langerhans cells (LCs). The LC 5-LO protein was largely found in the nuclear matrix, in nuclear envelopes, and perinuclear regions as indicated by
<jats:italic>in situ</jats:italic>
confocal laser scan microscopy. Reverse transcription–PCR and immunoblot analyses of purified primary EC populations further indicated that LCs are major 5-LO expressing cells. Enriched primary LCs were also found to contain 5-LO activating protein (FLAP), leukotriene (LT) C
<jats:sub>4</jats:sub>
synthase, and LTA
<jats:sub>4</jats:sub>
hydrolase. By contrast, 5-LO, FLAP, and LTC
<jats:sub>4</jats:sub>
synthase were undetectable or largely reduced, but LTA
<jats:sub>4</jats:sub>
hydrolase transcripts and protein were identified in ECs depleted of LCs. These data show that naïve LCs are major, and possibly the sole, 5-LO pathway expressing cells in the normal human epidermis.
</jats:p>