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Medientyp:
E-Artikel
Titel:
Evidence for anaphase pulling forces during C. elegans meiosis
Beteiligte:
Danlasky, Brennan M.;
Panzica, Michelle T.;
McNally, Karen P.;
Vargas, Elizabeth;
Bailey, Cynthia;
Li, Wenzhe;
Gong, Ting;
Fishman, Elizabeth S.;
Jiang, Xueer;
McNally, Francis J.
Erschienen:
Rockefeller University Press, 2020
Erschienen in:
Journal of Cell Biology, 219 (2020) 12
Sprache:
Englisch
DOI:
10.1083/jcb.202005179
ISSN:
0021-9525;
1540-8140
Entstehung:
Anmerkungen:
Beschreibung:
Anaphase chromosome movement is thought to be mediated by pulling forces generated by end-on attachment of microtubules to the outer face of kinetochores. However, it has been suggested that during C. elegans female meiosis, anaphase is mediated by a kinetochore-independent pushing mechanism with microtubules only attached to the inner face of segregating chromosomes. We found that the kinetochore proteins KNL-1 and KNL-3 are required for preanaphase chromosome stretching, suggesting a role in pulling forces. In the absence of KNL-1,3, pairs of homologous chromosomes did not separate and did not move toward a spindle pole. Instead, each homolog pair moved together with the same spindle pole during anaphase B spindle elongation. Two masses of chromatin thus ended up at opposite spindle poles, giving the appearance of successful anaphase.