Beschreibung:
<jats:title>Abstract</jats:title>
<jats:p>Motivation: Genetic screens by CRISPR/Cas9-mediated genome engineering have become a powerful tool for functional genomics. However, there is currently a lack of end-to-end software pipelines to analyze CRISPR/Cas9 screens based on next generation sequencing.</jats:p>
<jats:p>Results: The CRISPR-AnalyzeR for pooled screens (caRpools) is an R package for exploratory data analysis that provides a complete workflow to analyze CRISPR/Cas9 screens. To further support the analysis of large-scale screens, caRpools integrates screening documentation and generation of standardized analysis reports.</jats:p>
<jats:p>Availability and implementation: caRpools, manuals and an open virtual appliance are available at http://github.com/boutroslab/caRpools.</jats:p>
<jats:p>Contact: m.boutros@dkfz.de</jats:p>