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Medientyp:
E-Artikel
Titel:
Interference of soluble TNF-alpha receptors in immunological detection of tumor necrosis factor-alpha
Beteiligte:
Moreau, E;
Philippé, J;
Couvent, S;
Leroux-Roels, G
Erschienen:
Oxford University Press (OUP), 1996
Erschienen in:
Clinical Chemistry, 42 (1996) 9, Seite 1450-1453
Sprache:
Englisch
DOI:
10.1093/clinchem/42.9.1450
ISSN:
0009-9147;
1530-8561
Entstehung:
Anmerkungen:
Beschreibung:
Abstract We have studied the interference of soluble tumor necrosis factor-alpha receptor p55 (sTNF-R55) on the quantification of tumor necrosis factor-alpha (TNF-alpha) in three immunoassays: the commercially available enzyme amplified-sensitivity immunoassay (EASIA) of Medgenix and the ELISA of Boehringer Mannheim, and an assay based on electrochemiluminescence (ECL) technology. TNF-alpha measurements by EASIA and ELISA were not affected by the presence of sTNF-R55, but results by the ECL method were clearly influenced by the receptor. We then performed a second set of experiments with the ECL system to examine what factors might influence the importance of interference from sTNF-R55 and soluble TNF-alpha receptor p75 (sTNF-R75), which influences TNF-alpha measurements the same as sTNF-R55. We found that extending the incubation time and increasing the concentration of capture antibody decreased the interfering capacity of sTNF-R55 and sTNF-R75. A clear insight into these phenomena is of utmost importance for correct interpretation of cytokine quantification.