Chan, Louisa L Y;
Hui, Kenrie P Y;
Kuok, Denise I T;
Bui, Christine H T;
Ng, Ka-chun;
Mok, Chris K P;
Yang, Zi-feng;
Guan, Wenda;
Poon, Leo L M;
Zhong, Nanshan;
Peiris, J S Malik;
Nicholls, John M;
Chan, Michael C W
Risk Assessment of the Tropism and Pathogenesis of the Highly Pathogenic Avian Influenza A/H7N9 Virus Using Ex Vivo and In Vitro Cultures of Human Respiratory Tract
Sie können Bookmarks mittels Listen verwalten, loggen Sie sich dafür bitte in Ihr SLUB Benutzerkonto ein.
Medientyp:
E-Artikel
Titel:
Risk Assessment of the Tropism and Pathogenesis of the Highly Pathogenic Avian Influenza A/H7N9 Virus Using Ex Vivo and In Vitro Cultures of Human Respiratory Tract
Beteiligte:
Chan, Louisa L Y;
Hui, Kenrie P Y;
Kuok, Denise I T;
Bui, Christine H T;
Ng, Ka-chun;
Mok, Chris K P;
Yang, Zi-feng;
Guan, Wenda;
Poon, Leo L M;
Zhong, Nanshan;
Peiris, J S Malik;
Nicholls, John M;
Chan, Michael C W
Erschienen:
Oxford University Press (OUP), 2019
Erschienen in:
The Journal of Infectious Diseases, 220 (2019) 4, Seite 578-588
Beschreibung:
Abstract Background Highly pathogenic avian influenza (HPAI)-H7N9 virus arising from low pathogenic avian influenza (LPAI)-H7N9 virus with polybasic amino acid substitutions in the hemagglutinin was detected in 2017. Methods We compared the tropism, replication competence, and cytokine induction of HPAI-H7N9, LPAI-H7N9, and HPAI-H5N1 in ex vivo human respiratory tract explants, in vitro culture of human alveolar epithelial cells (AECs) and pulmonary microvascular endothelial cells (HMVEC-L). Results Replication competence of HPAI- and LPAI-H7N9 were comparable in ex vivo cultures of bronchus and lung. HPAI-H7N9 predominantly infected AECs, whereas limited infection was observed in bronchus. The reduced tropism of HPAI-H7N9 in bronchial epithelium may explain the lack of human-to-human transmission despite a number of mammalian adaptation markers. Apical and basolateral release of virus was observed only in HPAI-H7N9- and H5N1-infected AECs regardless of infection route. HPAI-H7N9, but not LPAI-H7N9 efficiently replicated in HMVEC-L. Conclusions Our findings demonstrate that a HPAI-H7N9 virus efficiently replicating in ex vivo cultures of human bronchus and lung. The HPAI-H7N9 was more efficient at replicating in human AECs and HMVEC-L than LPAI-H7N9 implying that endothelial tropism may involve in pathogenesis of HPAI-H7N9 disease.