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Medientyp:
E-Artikel
Titel:
pHBMT1, a BAHD-family monolignol acyltransferase, mediates lignin acylation in poplar
Beteiligte:
de Vries, Lisanne;
MacKay, Heather A;
Smith, Rebecca A;
Mottiar, Yaseen;
Karlen, Steven D;
Unda, Faride;
Muirragui, Emilia;
Bingman, Craig;
Vander Meulen, Kirk;
Beebe, Emily T;
Fox, Brian G;
Ralph, John;
Mansfield, Shawn D
Erschienen:
Oxford University Press (OUP), 2022
Erschienen in:Plant Physiology
Sprache:
Englisch
DOI:
10.1093/plphys/kiab546
ISSN:
0032-0889;
1532-2548
Entstehung:
Anmerkungen:
Beschreibung:
<jats:title>Abstract</jats:title>
<jats:p>Poplar (Populus) lignin is naturally acylated with p-hydroxybenzoate ester moieties. However, the enzyme(s) involved in the biosynthesis of the monolignol–p-hydroxybenzoates have remained largely unknown. Here, we performed an in vitro screen of the Populus trichocarpa BAHD acyltransferase superfamily (116 genes) using a wheatgerm cell-free translation system and found five enzymes capable of producing monolignol–p-hydroxybenzoates. We then compared the transcript abundance of the five corresponding genes with p-hydroxybenzoate concentrations using naturally occurring unrelated genotypes of P. trichocarpa and revealed a positive correlation between the expression of p-hydroxybenzoyl-CoA monolig-nol transferase (pHBMT1, Potri.001G448000) and p-hydroxybenzoate levels. To test whether pHBMT1 is responsible for the biosynthesis of monolignol–p-hydroxybenzoates, we overexpressed pHBMT1 in hybrid poplar (Populus alba × P. grandidentata) (35S::pHBMT1 and C4H::pHBMT1). Using three complementary analytical methods, we showed that there was an increase in soluble monolignol–p-hydroxybenzoates and cell-wall-bound monolignol–p-hydroxybenzoates in the poplar transgenics. As these pendent groups are ester-linked, saponification releases p-hydroxybenzoate, a precursor to parabens that are used in pharmaceuticals and cosmetics. This identified gene could therefore be used to engineer lignocellulosic biomass with increased value for emerging biorefinery strategies.</jats:p>