Beschreibung:
ObjectiveNeuropeptide calcitonin gene‐related peptide (CGRP) is a marker neurotransmitter related to vasculargensis in muscle differentiation and development of organ. The CGRP was first appeared at mouse embryonic day 16.5 (E16.5) and localization around blood vessels and closely cartilaginous bone matrix in the mouse limb (Bidegain et al., 1995). However, expression of CGRP and angiogenesis markers were unknown in developed mandible during craniofacial formation. Moreover, Osteopontin (OPN) detects in fibroblasts, preosteoblasts, osteoblasts, osteocytes, odontoblasts and bone marrow cells. We try to clearly the relation between CGRP, bone morphological marker, and angiogenesis marker in terminating the differentiation of mouse mandible.MethodsWe analyzed the expression and localization of CGRP, VEGF, OPN by in situ hybridization in the developing mouse mandible during embryonic days at E14.5 and E17.5. We also detected the mRNA abundance of various markers (Collagen I, VEGF, CD31, LYVE‐1, MMP‐2, OPN, and CGRP) from embryonic stages by real‐time RT‐PCR. Principal component analysis (PCA) was performed for mouse mandible in different early postnatal stages and the expression of the following 7 mRNA CGRP, OPN, CD31, LYVE‐1, VEGF, Collagen I, and MMP‐2.ResultsThe antisense probe for VEGF, OPN and CGRP was detected in mesenchymal cells surrounding area of mandible (Meckel's cartilage) at E14.5, and clearly localization was seen at E17.5 in the embryonic mouse masseter by in situ hybridization. These data suggested that angiogenesis markers may effect to influence during mouse Meckel's cartilage development and morphogenesis. These findings provide direct insight into the origins and potential prevalent development affecting the mandibular formation.Support or Funding Informationnone