Beschreibung:
Angiogenesis and osteogenesis markers are an important factor during development in relation between mandibular condyle and Meckel's cartilage. However, it has many functions in addition to the formation of mandible. We need to clearly the relationship between mandibular condyle and Meckel's cartilage to angiogenesis and osteogenesis markers during formation in mouse fetal and early postnatal stages. Therefore we try to investigate the correlation between these markers during the development of the mouse mandible. We analyzed the expression and localization of VEGF by in‐situ hybridization in the developing mouse mandibular condyle from embryonic days 12.5 to days 5. We also detected the mRNA abundance of mandible with various markers from embryonic and early postnatal stages by real‐time RT‐PCR. Our results was performed for mouse mandible in different postnatal stages and after birth and the expression of the following some mRNA markers (CD31, LYVE‐1, VEGF, collagen I, II, osteopontin, and osteocalcin). The antisense probe for VEGF was detected in mesenchymal cells surrounding around MC and bone matrices area from E17.5, and clearly localization was seen at E18.5 in the embryonic mouse mandible by in‐situ hybridization. These data suggested that angiogenesis markers were effected by development stages after or before birth in morphogenesis of mandible. VEGF also affects the other morphological markers in these specific stages terminating the differentiation in mouse mandibular condyle development.Support or Funding InformationNone