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Volti, Giovanni Li; Sorrenti, Valeria; Acquaviva, Rosaria; Murabito, Paolo; Gullo, Antonino; Barcellona, Maria Luisa; Galvano, Fabio; Rodella, Luigi; Rezzani, Rita; Vanella, Luca; Tringali, Giovanni; Caruso, Massimo; Gazzolo, Diego; Di Giacomo, Claudia

Effect of Ischemia–Reperfusion on Renal Expression and Activity of N  G-N  G-Dimethylarginine Dimethylaminohydrolases

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  • Medientyp: E-Artikel
  • Titel: Effect of Ischemia–Reperfusion on Renal Expression and Activity of N  G-N  G-Dimethylarginine Dimethylaminohydrolases
  • Beteiligte: Volti, Giovanni Li; Sorrenti, Valeria; Acquaviva, Rosaria; Murabito, Paolo; Gullo, Antonino; Barcellona, Maria Luisa; Galvano, Fabio; Rodella, Luigi; Rezzani, Rita; Vanella, Luca; Tringali, Giovanni; Caruso, Massimo; Gazzolo, Diego; Di Giacomo, Claudia
  • Erschienen: Ovid Technologies (Wolters Kluwer Health), 2008
  • Erschienen in: Anesthesiology
  • Sprache: Englisch
  • DOI: 10.1097/aln.0b013e31818d8a77
  • ISSN: 0003-3022
  • Schlagwörter: Anesthesiology and Pain Medicine
  • Entstehung:
  • Anmerkungen:
  • Beschreibung: <jats:sec> <jats:title>Background</jats:title> <jats:p>Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide synthase. It is degraded by the enzyme dimethylarginine dimethylaminohydrolase (DDAH).</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>Rats (n = 50) underwent to 45 min of renal ischemia followed by 30 min, 1 h, and 3 h of reperfusion. Expression of endothelial nitric oxide synthase, inducible nitric oxide synthase, DDAH-1, DDAH-2, renal DDAH activity, plasma NO2(-)/NO3(-), and ADMA levels were evaluated.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>Inducible nitric oxide synthase expression increased, as confirmed by both plasma (11.89 +/- 1.02, 15.56 +/- 0.93, 11.82 +/- 0.86, 35.05 +/- 1.28, and 43.89 +/- 1.63 nmol/ml in the control, ischemic, 30-min, 1-h, and 3-h groups, respectively) and renal (4.81 +/- 0.4, 4.85 +/- 1, 9.42 +/- 0.7, 15.42 +/- 0.85, and 22.03 +/- 1.11 nmol/mg protein) formations of NO2(-)/NO3(-). DDAH-1 expression decreased after reperfusion, whereas DDAH-2 increased after 30 min, returning to basal levels after 3 h. Total DDAH activity was reduced during all times of reperfusion. Both plasma (0.41 +/- 0.03, 0.43 +/- 0.05, 0.62 +/- 0.02, 0.71 +/- 0.02, and 0.41 +/- 0.01 nmol/ml in the control, ischemic, 30-min, 1-h, and 3-h groups, respectively) and renal (1.51 +/- 0.01, 1.5 +/- 0.01, 1.53 +/- 0.01, 2.52 +/- 0.04, and 4.48 +/- 0.03 nmol/mg protein in the control, ischemic, 30-min, 1-h, and 3-h groups, respectively) concentrations of ADMA increased.</jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions</jats:title> <jats:p>Results suggest that ischemia-reperfusion injury leads to reduced DDAH activity and modification of different DDAH isoform expression, thus leading to increased ADMA levels, which may lead to increased cardiovascular risk.</jats:p> </jats:sec>
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