Erschienen in:International Journal of Systematic and Evolutionary Microbiology
Sprache:
Englisch
DOI:
10.1099/ijs.0.019927-0
ISSN:
1466-5034;
1466-5026
Entstehung:
Anmerkungen:
Beschreibung:
<jats:p>A non-lipophilic, coryneform bacterium isolated from a patient's wound caused by a dog bite was characterized by phenotypic, chemotaxonomic and molecular genetic methods. Chemotaxonomic features suggested assignment of the unknown bacterium to the genus <jats:italic>Corynebacterium</jats:italic>. The isolate exhibited the following unusual features, which made it possible to phenotypically differentiate it from all other medically relevant corynebacteria: the Gram stain showed some very filamentous rods (>15 μm in length); some cells exhibited branching; colonies were domed and adherent to agar; the micro-organism was positive for pyrazinamidase, <jats:italic>β</jats:italic>-glucosidase, <jats:italic>α</jats:italic>-glucosidase and trypsin but negative for <jats:italic>β</jats:italic>-galactosidase. 16S rRNA gene sequencing and partial <jats:italic>rpoB</jats:italic> gene sequencing showed that the closest phylogenetic relative, <jats:italic>Corynebacterium freiburgense</jats:italic>, exhibited more than 1.9 % and 17.9 % divergence with the unknown bacterium, respectively. Based on both phenotypic and molecular genetic data, it is proposed that the isolate should be classified as a novel species, <jats:italic>Corynebacterium canis</jats:italic> sp. nov., with the type strain 1170<jats:sup>T</jats:sup> (=CCUG 58627<jats:sup>T</jats:sup> =DSM 45402<jats:sup>T</jats:sup>).</jats:p>