Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse

Medientyp: E-Artikel
Titel: Sporofaciens musculi gen. nov., sp. nov., a novel bacterium isolated from the caecum of an obese mouse
Beteiligte: Rasmussen, Torben Sølbeck; Streidl, Theresa; Hitch, Thomas C. A.; Wortmann, Esther; Deptula, Paulina; Kofoed, Michael V. W.; Riedel, Thomas; Neumann-Schaal, Meina; Hansen, Michael; Nielsen, Dennis Sandris; Clavel, Thomas; Vogensen, Finn Kvist
Erschienen: Microbiology Society, 2019
Erschienen in: International Journal of Systematic and Evolutionary Microbiology
Sprache: Englisch
DOI: 10.1099/ijsem.0.004673
ISSN: 1466-5026; 1466-5034
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Beschreibung: <jats:p>A bacterial strain, designated WCA-9-b2<jats:sup>T</jats:sup>, was isolated from the caecal content of an 18-week-old obese C57BL/6NTac male mouse. According to phenotypic analyses, the isolate was rod-shaped, strictly anaerobic, spore-forming, non-motile and Gram-stain-positive, under the conditions tested. Colonies were irregular and non-pigmented. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the order<jats:italic><jats:named-content content-type="order"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3876" xlink:type="simple">Clostridiales</jats:ext-link></jats:named-content></jats:italic>with<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4087" xlink:type="simple">Dorea longicatena</jats:ext-link></jats:named-content></jats:italic>ATCC 27755<jats:sup>T</jats:sup>(94.9 % sequence identity),<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4156" xlink:type="simple">Ruminococcus gnavus</jats:ext-link></jats:named-content></jats:italic>ATCC 29149<jats:sup>T</jats:sup>(94.8%) and<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4016" xlink:type="simple">Clostridium scindens</jats:ext-link></jats:named-content></jats:italic>ATCC 35704<jats:sup>T</jats:sup>(94.3%) being the closest relatives. Whole genome sequencing showed an average nucleotide identity <74.23 %, average amino acid identity <64.52–74.67 % and percentage of conserved proteins values <50 % against the nine closest relatives (<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4087" xlink:type="simple">D. longicatena</jats:ext-link></jats:named-content></jats:italic>,<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4156" xlink:type="simple">Ruminococcus gnavus</jats:ext-link></jats:named-content></jats:italic>,<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4016" xlink:type="simple">C. scindens</jats:ext-link></jats:named-content></jats:italic>,<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4086" xlink:type="simple">Dorea formicigenerans</jats:ext-link></jats:named-content></jats:italic>,<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4159" xlink:type="simple">Ruminococcus lactaris</jats:ext-link></jats:named-content></jats:italic>,<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3949" xlink:type="simple">Clostridium hylemonae</jats:ext-link></jats:named-content></jats:italic>,<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.30404" xlink:type="simple">Merdimonas faecis</jats:ext-link></jats:named-content></jats:italic>,<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.29880" xlink:type="simple">Faecalicatena contorta</jats:ext-link></jats:named-content></jats:italic>and<jats:italic><jats:named-content content-type="species"><jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.29881" xlink:type="simple">Faecalicatena fissicatena</jats:ext-link></jats:named-content></jats:italic>). The genome-based G+C content of genomic DNA was 44.4 mol%. The major cellular fatty acids were C<jats:sub>16 : 0</jats:sub>(24.5%), C<jats:sub>18 : 1</jats:sub><jats:italic>cis</jats:italic>9 (19.8 %), C<jats:sub>16 : 0</jats:sub>DMA (11.7%), C<jats:sub>18 : 0</jats:sub>(8.4%) and C<jats:sub>14 : 0</jats:sub>(6.6%). Respiratory quinones were not detected. The predominant metabolic end products of glucose fermentation were acetate and succinate. Production of CO<jats:sub>2</jats:sub>and H<jats:sub>2</jats:sub>were detected. Based on these data, we propose that strain WCA-9-b2<jats:sup>T</jats:sup>represents a novel species within a novel genus, for which the name<jats:italic>Sporofaciens musculi</jats:italic>gen. nov., sp. nov. is proposed. The type strain is WCA-9-b2<jats:sup>T</jats:sup>(=DSM 106039<jats:sup>T</jats:sup>=CECT 30156<jats:sup>T</jats:sup>).</jats:p>
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