Erschienen in:
International Journal of Systematic and Evolutionary Microbiology, 72 (2022) 5
Sprache:
Englisch
DOI:
10.1099/ijsem.0.005387
ISSN:
1466-5026;
1466-5034
Entstehung:
Anmerkungen:
Beschreibung:
Seven bacterial strains isolated from bovine endocarditis in six animals from different geographic regions were investigated in a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences placed all seven isolates on a distinct, monophyletic cluster in the familyNeisseriaceaewith closest similarity to type strains ofAlysiella filiformis(97.06 %) andKingella kingae(96.34 %). Whole genome sequence analysis of isolates confirmed their species status, with an average nucleotide identity >96 % between isolates and <80 % to other type species of genera ofNeisseriaceaewhile digital DNA–DNA hybridization values were >80 % and<18 %, respectively. The DNA G+C content was 42.5–43.0 mol%. Whole genome sequence based phylogeny showed the isolates being monophyletic and separated from established genera, thereby forming a new genus within the familyNeisseriaceae. Similarly, analysis of MALDI-TOF MS reference spectra clustered the isolates close together and clearly separated from other genera, making this the method of choice for identification. Biochemical markers based on classical as well as commercial identification schemes allowed separation from closely relatedNeisseriaceaegenera, even though the new taxon is biochemically not very active. Major fatty acids are C12 : 0, C14 : 0and C16 : 0. The major quinone is ubiquinone Q-8. In the polar lipid profile, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phospholipid were predominant. We propose the novel genusWielerellawith the type speciesWielerella bovisgen. nov., sp. nov. The type strain is CCUG 44465T(=DSM 113289T=JF 2483T) isolatedpost mortemfrom a cow with endocarditis in Switzerland.