Luminal and mucosa-associated caecal microbiota of chickens after experimental Campylobacter jejuni infection in the absence of Campylobacter-specific phages of group II and III

Medientyp: E-Artikel
Titel: Luminal and mucosa-associated caecal microbiota of chickens after experimental Campylobacter jejuni infection in the absence of Campylobacter-specific phages of group II and III
Beteiligte: Hankel, Julia; Kittler, Sophie; Chuppava, Bussarakam; Galvez, Eric; Strowig, Till; Becker, André; von Köckritz-Blickwede, Maren; Plötz, Madeleine; Visscher, Christian
Erschienen: Microbiology Society, 2022
Erschienen in: Microbial Genomics, 8 (2022) 10
Sprache: Englisch
DOI: 10.1099/mgen.0.000874
ISSN: 2057-5858
Schlagwörter: General Medicine
Entstehung:
Anmerkungen:
Beschreibung: <jats:p>Campylobacteriosis is still the most commonly reported zoonosis in the European Union causing gastrointestinal disease in humans. One of the most common sources for these food-borne infections is broiler meat. Interactions between <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3786" xlink:type="simple">Campylobacter</jats:ext-link> </jats:named-content> </jats:italic> (<jats:italic>C</jats:italic>.) <jats:italic>jejuni</jats:italic> and the intestinal microbiota might influence <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3786" xlink:type="simple">Campylobacter</jats:ext-link> </jats:named-content> </jats:italic> colonization in chickens. The aim of the present study was to gain further knowledge about exclusive interactions of the host microbiota with <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10649" xlink:type="simple">C. jejuni</jats:ext-link> </jats:named-content> </jats:italic> in <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3786" xlink:type="simple">Campylobacter</jats:ext-link> </jats:named-content> </jats:italic>-specific phage-free chickens under standardized conditions and special biosafety precautions.</jats:p> <jats:p>Therefore, 12 artificially infected (<jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10649" xlink:type="simple">C. jejuni</jats:ext-link> </jats:named-content> </jats:italic> inoculum with a challenge dose of 7.64 log<jats:sub>10</jats:sub> c.f.u.) and 12 control chickens of the breed Ross 308 were kept under special biosafety measures in an animal facility. At day 42 of life, microbiota studies were performed on samples of caecal digesta and mucus. No <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3786" xlink:type="simple">Campylobacter</jats:ext-link> </jats:named-content> </jats:italic>-specific phages were detected by real-time PCR analysis of caecal digesta of control or artificially infected chickens. Amplification of the 16S rRNA gene was performed within the hypervariable region V4 and subsequently sequenced with Illumina MiSeq platform. R (version 4.0.2) was used to compare the microbiota between <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10649" xlink:type="simple">C. jejuni</jats:ext-link> </jats:named-content> </jats:italic>-negative and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10649" xlink:type="simple">C. jejuni</jats:ext-link> </jats:named-content> </jats:italic>-positive chickens. The factor chickens’ infection status contributed significantly to the differences in microbial composition of mucosal samples, explaining 10.6 % of the microbiota variation (<jats:italic>P</jats:italic>=0.007) and in digesta samples, explaining 9.69 % of the microbiota variation (<jats:italic>P</jats:italic>=0.015). The strongest difference between <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10649" xlink:type="simple">C. jejuni</jats:ext-link> </jats:named-content> </jats:italic>-non-infected and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10649" xlink:type="simple">C. jejuni</jats:ext-link> </jats:named-content> </jats:italic>-infected birds was observed for the family <jats:italic> <jats:named-content content-type="family"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.4303" xlink:type="simple">Peptococcaceae</jats:ext-link> </jats:named-content> </jats:italic> whose presence in <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10649" xlink:type="simple">C. jejuni</jats:ext-link> </jats:named-content> </jats:italic>-infected birds could not be demonstrated. Further, several genera of the family <jats:italic> <jats:named-content content-type="family"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.14008" xlink:type="simple">Ruminococcaceae</jats:ext-link> </jats:named-content> </jats:italic> appeared to be depressed in its abundance due to <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3786" xlink:type="simple">Campylobacter</jats:ext-link> </jats:named-content> </jats:italic> infection. A negative correlation was found between <jats:italic> <jats:named-content content-type="family"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.22787" xlink:type="simple">Christensenellaceae</jats:ext-link> </jats:named-content> </jats:italic> R-7 group and <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3786" xlink:type="simple">Campylobacter</jats:ext-link> </jats:named-content> </jats:italic> in <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.10649" xlink:type="simple">C. jejuni</jats:ext-link> </jats:named-content> </jats:italic>-colonised chickens, both genera potentially competing for substrate. This makes <jats:italic> <jats:named-content content-type="family"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.22787" xlink:type="simple">Christensenellaceae</jats:ext-link> </jats:named-content> </jats:italic> R-7 group highly interesting for further studies that aim to find control options for <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3786" xlink:type="simple">Campylobacter</jats:ext-link> </jats:named-content> </jats:italic> infections and assess the relevance of this finding for chicken health and <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3786" xlink:type="simple">Campylobacter</jats:ext-link> </jats:named-content> </jats:italic> colonization.</jats:p>
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